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Literature summary extracted from

  • Liu, J.; Liu, X.; Shi, Y.
    Expression, purification, and characterization of alanine racemase from Pseudomonas putida YZ-26 (2012), World J. Microbiol. Biotechnol., 28, 267-274.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.1 expressed in Escherichia coli BL21 cells Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.1 Cu2+ 40% residual activity at 1 mM Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.1 10.34
-
L-alanine at pH 9.0 and 37°C Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.1.1 Ca2+ slight activation at 1 mM Pseudomonas putida
5.1.1.1 Co2+ 120% activity at 1 mM Pseudomonas putida
5.1.1.1 Fe2+ slight activation at 1 mM Pseudomonas putida
5.1.1.1 Mg2+ slight activation at 1 mM Pseudomonas putida
5.1.1.1 Mn2+ 130% activity at 1 mM Pseudomonas putida
5.1.1.1 additional information not influenced by 1 mM Zn2+ or Sn2+ Pseudomonas putida
5.1.1.1 Ni2+ slight activation at 1 mM Pseudomonas putida
5.1.1.1 Sr2+ 158% activity at 1 mM Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.1 42370
-
gel filtration Pseudomonas putida
5.1.1.1 43000
-
1 * 43000, SDS-PAGE Pseudomonas putida
5.1.1.1 44217
-
1 * 44217, calculated from amino acid sequence Pseudomonas putida
5.1.1.1 44220
-
calculated from amino acid sequence Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.1 Pseudomonas putida
-
-
-
5.1.1.1 Pseudomonas putida YZ-26
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.1 Ni2+-NTA column chromatography and Sephacryl S-200 gel filtration Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.1 11.5
-
crude extract, using L-alanine as substrate, at pH 9.0 and 37°C Pseudomonas putida
5.1.1.1 16.5
-
after 3.7fold purification, using L-isoleucine as substrate, at pH 9.0 and 37°C Pseudomonas putida
5.1.1.1 42.6
-
after 3.7fold purification, using L-alanine as substrate, at pH 9.0 and 37°C Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.1 L-alanine optimal substrate for alanine racemase Pseudomonas putida D-alanine
-
?
5.1.1.1 L-alanine optimal substrate for alanine racemase Pseudomonas putida YZ-26 D-alanine
-
?
5.1.1.1 L-isoleucine
-
Pseudomonas putida D-isoleucine
-
?
5.1.1.1 L-isoleucine
-
Pseudomonas putida YZ-26 D-isoleucine
-
?
5.1.1.1 additional information the enzyme is only specific to L-alanine and L-isoleucine, and does not catalyze isomerization the other amino acids Pseudomonas putida ?
-
?
5.1.1.1 additional information the enzyme is only specific to L-alanine and L-isoleucine, and does not catalyze isomerization the other amino acids Pseudomonas putida YZ-26 ?
-
?

Subunits

EC Number Subunits Comment Organism
5.1.1.1 monomer 1 * 43000, SDS-PAGE Pseudomonas putida
5.1.1.1 monomer 1 * 44217, calculated from amino acid sequence Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
5.1.1.1 ALR
-
Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.1 37
-
-
Pseudomonas putida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.1.1.1 25 50
-
Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.1.1 25 80 95% activity at 25°C, 80% activity at 40°C, less than 20% activity at 50°C, less than 10% activity at 60 and 70°C, inactive at 80°C Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.1.1 70.63
-
L-alanine at pH 9.0 and 37°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.1 9
-
-
Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.1.1 7 11 when the enzyme is incubated at 37°C in 100 mM sodium acetate, 100 mM sodium phosphate, 100 mM Tris/HCl, and 100 mM borate/NaOH buffer for 30 min, more than 80% of the initial activity is retained at pH 7.0-11.0 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.1 pyridoxal 5'-phosphate
-
Pseudomonas putida