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Literature summary extracted from

  • Park, C.; Yeom, S.; Lim, Y.; Kim, Y.; Oh, D.
    Substrate specificity of a recombinant ribose-5-phosphate isomerase from Streptococcus pneumoniae and its application in the production of l-lyxose and l-tagatose (2011), World J. Microbiol. Biotechnol., 27, 743-750.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.3.1.6 synthesis RpiB is a potential producer of L-form monosaccharides Streptococcus pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.6 expressed in Escherichia coli ER2566 cells Streptococcus pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.6 additional information the enzyme is not activated by monovalent or divalent cations Streptococcus pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.6 23724
-
 4 * 23724, calculated from amino acid sequence Streptococcus pneumoniae
5.3.1.6 24000
-
 4 * 24000, SDS-PAGE Streptococcus pneumoniae
5.3.1.6 96000
-
 gel filtration Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.6 D-ribose 5-phosphate Streptococcus pneumoniae highest specific activity D-ribulose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.6 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.6 HiTrap Q anion exchange column chromatography and Sephacryl S-300 gel filtration Streptococcus pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.6 0.0005
-
 using D-sorbose as substrate, at 35°C and pH 7.5 Streptococcus pneumoniae
5.3.1.6 0.0011
-
 using L-fructose as substrate, at 35°C and pH 7.5 Streptococcus pneumoniae
5.3.1.6 0.0013
-
 using L-tagatose as substrate, at 35°C and pH 7.5 Streptococcus pneumoniae
5.3.1.6 0.0019
-
 using D-allose as substrate, at 35°C and pH 7.5 Streptococcus pneumoniae
5.3.1.6 0.0021
-
 using D-psicose as substrate, at 35°C and pH 7.5 Streptococcus pneumoniae
5.3.1.6 0.0025
-
 using D-ribose as substrate, at 35°C and pH 7.5 Streptococcus pneumoniae
5.3.1.6 0.0053
-
 using D-gulose as substrate, at 35°C and pH 7.5 Streptococcus pneumoniae
5.3.1.6 0.0096
-
 using L-lyxose as substrate, at 35°C and pH 7.5 Streptococcus pneumoniae
5.3.1.6 0.019
-
 using L-talose as substrate, at 35°C and pH 7.5 Streptococcus pneumoniae
5.3.1.6 0.027
-
 using L-lyxose as substrate, at 35°C and pH 7.5 Streptococcus pneumoniae
5.3.1.6 0.048
-
 using D-ribulose as substrate, at 35°C and pH 7.5 Streptococcus pneumoniae
5.3.1.6 0.058
-
 using D-glucose 6-phosphate as substrate, at 35°C and pH 7.5 Streptococcus pneumoniae
5.3.1.6 0.121
-
 using L-xylulose as substrate, at 35°C and pH 7.5 Streptococcus pneumoniae
5.3.1.6 0.388
-
 using D-ribose 5-phosphate as substrate, at 35°C and pH 7.5 Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.6 D-allose
-
 Streptococcus pneumoniae D-psicose
-
 r
5.3.1.6 D-Glucose 6-phosphate
-
 Streptococcus pneumoniae ?
-
 ?
5.3.1.6 D-gulose
-
 Streptococcus pneumoniae D-sorbose
-
 r
5.3.1.6 D-psicose
-
 Streptococcus pneumoniae D-allose
-
 r
5.3.1.6 D-ribose
-
 Streptococcus pneumoniae D-ribulose
-
 r
5.3.1.6 D-ribose 5-phosphate highest specific activity Streptococcus pneumoniae D-ribulose 5-phosphate
-
 ?
5.3.1.6 D-ribulose
-
 Streptococcus pneumoniae D-ribose
-
 r
5.3.1.6 D-sorbose lowest activity with L-sorbose Streptococcus pneumoniae D-gulose
-
 r
5.3.1.6 L-fructose
-
 Streptococcus pneumoniae L-mannose
-
 r
5.3.1.6 L-lyxose particularly high activity with L-lyxose Streptococcus pneumoniae L-xylulose
-
 r
5.3.1.6 L-Mannose
-
 Streptococcus pneumoniae L-Fructose
-
 r
5.3.1.6 L-tagatose particularly high activity with L-tagatose Streptococcus pneumoniae L-talose
-
 r
5.3.1.6 L-talose particularly high activity with L-talose Streptococcus pneumoniae L-tagatose
-
 r
5.3.1.6 L-xylulose particularly high activity with L-lyxose Streptococcus pneumoniae L-lyxose
-
 r
5.3.1.6 additional information no activity is observed with D-mannose-6-phosphate Streptococcus pneumoniae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.6 homotetramer 4 * 24000, SDS-PAGE Streptococcus pneumoniae
5.3.1.6 homotetramer 4 * 23724, calculated from amino acid sequence Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
5.3.1.6 ribose-5-phosphate isomerase
-
 Streptococcus pneumoniae
5.3.1.6 RpiB
-
 Streptococcus pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.6 35
-
-
 Streptococcus pneumoniae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.6 20 45
-
 Streptococcus pneumoniae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.6 35 50 enzyme activity is stable for 48 h at 35°C, and shows half-lives of 15 h at 40°C, and 6 h at 50°C Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.6 7.5
-
-
 Streptococcus pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.6 6.5 8.5
-
 Streptococcus pneumoniae