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Literature summary extracted from

  • Mehra-Chaudhary, R.; Mick, J.; Tanner, J.J.; Henzl, M.T.; Beamer, L.J.
    Crystal structure of a bacterial phosphoglucomutase, an enzyme involved in the virulence of multiple human pathogens (2011), Proteins, 79, 1215-1229.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.2 expressed in Escherichia coli BL21(DE3) cells Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.2.2 hanging drop vapor diffusion method, using 0.1 M Bis-Tris, pH 6.5, 0.2 M MgCl2, and 25% (w/v) PEG 3350 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.2 Mn2+ a single Mg2+ ion is required for catalytic activity, each PGM monomer contains one bound Mg2+ ion in its metal binding site Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.2.2 60800
-
 2 * 60800, calculated from amino acid sequence Salmonella enterica subsp. enterica serovar Typhimurium
5.4.2.2 105200
-
 dynamic light scattering Salmonella enterica subsp. enterica serovar Typhimurium
5.4.2.2 109600
-
 analytical ultracentrifugation Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.2 Salmonella enterica subsp. enterica serovar Typhimurium Q8ZQW9 serovar typhimurium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.2 His-Select Ni-Affinity gel column chromatography, gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium alpha-D-glucose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.2.2 homodimer 2 * 60800, calculated from amino acid sequence Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
5.4.2.2 PGM
-
 Salmonella enterica subsp. enterica serovar Typhimurium