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Literature summary extracted from

  • Orita, I.; Kita, A.; Yurimoto, H.; Kato, N.; Sakai, Y.; Miki, K.
    Crystal structure of 3-hexulose-6-phosphate synthase, a member of the orotidine 5-monophosphate decarboxylase suprafamily (2010), Proteins Struct. Funct. Bioinform., 78, 3488-3492.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.43 expressed in Escherichia coli Rosetta (DE3) cells Mycobacterium gastri

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.43 in complex with a sulfate ion, sitting drop vapor diffusion method, using 18% (w/v) polyethylene glycol (PEG) 3350 and 0.2 M MgCl2 Mycobacterium gastri

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.43 H134A the mutation abolishes HPS activity Mycobacterium gastri
4.1.2.43 H134D the mutation abolishes HPS activity Mycobacterium gastri
4.1.2.43 H134K the mutation abolishes HPS activity Mycobacterium gastri
4.1.2.43 H134N the mutation abolishes HPS activity Mycobacterium gastri
4.1.2.43 H134S the mutation abolishes HPS activity Mycobacterium gastri
4.1.2.43 K61A the mutant exhibits 50-90% of the activity of the wild type enzyme Mycobacterium gastri
4.1.2.43 K61D the mutant exhibits 50-90% of the activity of the wild type enzyme Mycobacterium gastri
4.1.2.43 K61H the mutant exhibits 50-90% of the activity of the wild type enzyme Mycobacterium gastri
4.1.2.43 K61S the mutant exhibits 50-90% of the activity of the wild type enzyme Mycobacterium gastri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.43 1.49
-
 D-ribulose 5-phosphate pH and temperature not specified in the publication Mycobacterium gastri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.43 Mg2+ the enzyme requires an Mg2+-assisted formation of an enediolate intermediate for catalysis of its reaction. The active site of each HPS monomer contains a magnesium ion Mycobacterium gastri
4.1.2.43 sulfate the active site of each HPS monomer contains a sulfate ion Mycobacterium gastri

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.43 Mycobacterium gastri Q9LBW4
-
-
4.1.2.43 Mycobacterium gastri MB19 Q9LBW4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.43
-
 Mycobacterium gastri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Mycobacterium gastri D-arabino-hex-3-ulose 6-phosphate
-
 ?
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Mycobacterium gastri MB19 D-arabino-hex-3-ulose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.43 dimer x-ray crystallography Mycobacterium gastri

Synonyms

EC Number Synonyms Comment Organism
4.1.2.43 HPS
-
 Mycobacterium gastri