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Literature summary extracted from

  • Park, J.; Dias, C.; Lee, S.; Valentini, S.; Sokabe, M.; Fraser, C.; Park, M.
    Production of active recombinant eIF5A: Reconstitution in E. coli of eukaryotic hypusine modification of eIF5A by its coexpression with modifying enzymes (2011), Protein Eng. Des. Sel., 24, 301-309.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.29 construction of a polycistronic vector encoding eIF5A, DHS and DOHH, expression in Escherichia coli strain BL21(DE3), overview. Effects of time and temperature on the expression of recombinant proteins and hypusine modification, DOHH effectively hydroxylates all the eIF5A(Dhp), a deoxyhypusine-containing intermediate, formed Homo sapiens
2.5.1.46 DHS DNA and amino acid seuence determination and analysis, functional coexpression with human deoxyhypusine hydroxylase and human eukaryotic translation initiation factor 5A in Escherichia coli strain BL21(DE3)pLys that produces eIF5A(Hpu), overview Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.99.29 additional information construction of a polycistronic vector encoding eIF5A, DHS and DOHH, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.29 eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2 Homo sapiens the DOHH reaction is irreversible eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
 ir
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Homo sapiens
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.29 Homo sapiens
-
-
-
2.5.1.46 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.29 eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
-
 Homo sapiens eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
 ir
1.14.99.29 eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2 the DOHH reaction is irreversible Homo sapiens eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
 ir
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Homo sapiens [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.29 deoxyhypusine hydroxylase
-
 Homo sapiens
1.14.99.29 DOHH
-
 Homo sapiens
2.5.1.46 DHS
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
1.14.99.29 evolution hypusine occurs only in eukaryotes and certain archaea, but not in eubacteria Homo sapiens
1.14.99.29 metabolism eukaryotic translation initiation factor 5A, eIF5A, is the only cellular protein that contains the polyamine-modified lysine, hypusine, i.e. N6-(4-amino-2-hydroxybutyl)lysine, which is formed post-translationally by two consecutive enzymatic reactions catalyzed by deoxyhypusine synthase, DHS, and deoxyhypusine hydroxylase, DOHH Homo sapiens
1.14.99.29 physiological function Hypusine modification is essential for the activity of eIF5A and for eukaryotic cell proliferation. eIF5A binds to the ribosome and stimulates translation in a hypusine-dependent manner Homo sapiens
2.5.1.46 metabolism deoxyhypusine synthase catalyzes a step in hypusine sythesis, a unique mmoified lysine derivative of eukaryotic translation initiation factor 5A, eIF5A Homo sapiens
2.5.1.46 physiological function eukaryotic translation initiation factor 5A, eIF5A, is a small acidic protein and the only cellular protein that contains the unique modified lysine, hypusine [N1-(4-amino-2-hydroxybutyl)lysine]. Hypusine is formed by the post-translational modification of one specific lysine residue of the eIF5A precursor, eIF5A(Lys), in two consecutive enzymatic steps. The first enzyme, deoxyhypusine synthase, DHS, catalyzes the transfer of the aminobutyl moiety from the polyamine spermidine to form an intermediate, deoxyhypusine [N1-(4-aminobutyl)lysine] residue, which is subsequently hydroxylated by deoxyhypusine hydroxylase Homo sapiens