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Literature summary extracted from

  • Yoshida, H.; Takeda, K.; Izumori, K.; Kamitori, S.
    Elucidation of the role of Ser329 and the C-terminal region in the catalytic activity of Pseudomonas stutzeri L-rhamnose isomerase (2010), Protein Eng. Des. Sel., 23, 919-927.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.14 wild type and mutant enzymes S329F, S329K, S329L, and S329A in complex with L-rhamnose, D-psicose or D-allose, droplet vapor diffusion method, using 6-9.25% (w/v) polyethylene glycol 20000, 50 mM MES buffer, pH 6.1-6.3, at 20°C Pseudomonas stutzeri

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.14 S329A mutant showing reduced catalytic efficiency compared to the wild type enzyme Pseudomonas stutzeri
5.3.1.14 S329F mutant showing reduced catalytic efficiency compared to the wild type enzyme Pseudomonas stutzeri
5.3.1.14 S329K mutant showing reduced catalytic efficiency compared to the wild type enzyme Pseudomonas stutzeri
5.3.1.14 S329L mutant showing reduced catalytic efficiency compared to the wild type enzyme Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.14 7.9
-
 L-rhamnose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 12.2
-
 L-rhamnose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 18.5
-
 L-rhamnose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 19.4
-
 L-rhamnose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 24.2
-
 L-rhamnose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 25.9
-
 D-allose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 33.9
-
 D-allose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 35.6
-
 D-allose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 39.1
-
 D-ribose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 44.4
-
 D-ribose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 44.7
-
 D-allose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 53.3
-
 D-allose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 65.6
-
 D-ribose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 67.3
-
 D-ribose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 85.1
-
 D-ribose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.14 Pseudomonas stutzeri Q75WH8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.14
-
 Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.14 D-allose
-
 Pseudomonas stutzeri D-psicose
-
 r
5.3.1.14 D-ribose
-
 Pseudomonas stutzeri D-ribulose
-
 r
5.3.1.14 L-Rhamnose
-
 Pseudomonas stutzeri L-Rhamnulose
-
 r
5.3.1.14 additional information the enzyme recognizes substrates at C2 and C3 but not at C4 and C5 Pseudomonas stutzeri ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.14 L-RhI
-
 Pseudomonas stutzeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.14 1.7
-
 D-ribose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 1.8
-
 D-ribose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 2.7
-
 D-allose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 3.3
-
 D-ribose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 3.8
-
 D-allose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 5.1
-
 L-rhamnose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 7.4
-
 D-allose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 8.9
-
 D-ribose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 9.8
-
 D-allose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 11.4
-
 D-ribose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 13.3
-
 L-rhamnose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 21.2
-
 D-allose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 53.6
-
 L-rhamnose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 142
-
 L-rhamnose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 224
-
 L-rhamnose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.14 0.0027
-
 D-ribose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 0.0043
-
 D-ribose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 0.005
-
 D-ribose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 0.071
-
 D-allose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 0.081
-
 D-allose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 0.13
-
 D-ribose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 0.2
-
 D-ribose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 0.27
-
 D-allose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 0.28
-
 D-allose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 0.41
-
 L-rhamnose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 0.47
-
 D-allose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 0.55
-
 L-rhamnose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 6.8
-
 L-rhamnose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 7.7
-
 L-rhamnose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.3.1.14 11.6
-
 L-rhamnose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri