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Literature summary extracted from

  • Myung, S.; Wang, Y.; Zhang, Y.
    Fructose-1,6-bisphosphatase from a hyper-thermophilic bacterium Thermotoga maritima: characterization, metabolite stability, and its implications (2010), Process Biochem., 45, 1882-1887.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11 expression as cellulose-binding module-intein-fusion protein in Escherichia coli strain BL21 Star (DE3) Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.04
-
 D-fructose 1,6-bisphosphate pH 7.5, 60°C, purified recombinant enzyme Thermotoga maritima
3.1.3.11 0.12
-
 D-fructose 1,6-bisphosphate pH 7.5, 80°C, purified recombinant enzyme Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Mg2+ required Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 28000
-
 x * 28000, SDS-PAGE Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Thermotoga maritima
-
 D-fructose 6-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11 recombinant cellulose-binding module-intein-fusion enzyme 5.62fold from Escherichia coli strain BL21 Star (DE3) by affinity chromatography on regenerated amorphous cellulose followed by intein self-cleavage Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.11 12.13
-
 pH 7.5, 60°C, purified recombinant enzyme Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Thermotoga maritima D-fructose 6-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.11 ? x * 28000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.1.3.11 FBPase
-
 Thermotoga maritima
3.1.3.11 fructose-1,6-bisphosphatase
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.11 80
-
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.11 60
-
 purified recombinant enzyme, half-life is 1330 h Thermotoga maritima
3.1.3.11 80
-
 purified recombinant enzyme, half-life is 55.6 h Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.11 8.57
-
 D-fructose 1,6-bisphosphate pH 7.5, 60°C, purified recombinant enzyme Thermotoga maritima
3.1.3.11 58.7
-
 D-fructose 1,6-bisphosphate pH 7.5, 80°C, purified recombinant enzyme Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.11 7.5
-
 assay at Thermotoga maritima