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Literature summary extracted from

  • Alte, F.; Stengel, A.; Benz, J.P.; Petersen, E.; Soll, J.; Groll, M.; Boelter, B.
    Ferredoxin:NADPH oxidoreductase is recruited to thylakoids by binding to a polyproline type II helix in a pH-dependent manner (2010), Proc. Natl. Acad. Sci. USA, 107, 19260-19265.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.18.1.2 analysis of the enzyme in complex with a synthetic peptide representing the first FNR-binding repeat from Pisum sativum Tic62, including the strictly conserved core motif KTEQPLSPYTAYDDLKPPSSPSPTKPS, based on the crystal structure PDB ID 1QG0, X-ray diffraction structure analysis at 1.7 A resolution, molecular replacement Pisum sativum

General Stability

EC Number General Stability Organism
1.18.1.2 interaction with Tic62 stabilizes FNR Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.18.1.2 chloroplast FNR is a soluble protein associated with thylakoid membranes Pisum sativum 9507
-
1.18.1.2 thylakoid membrane FNR is a soluble protein associated with thylakoid membranes Pisum sativum 42651
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.2 2 reduced ferredoxin + NADP+ Pisum sativum
-
 2 oxidized ferredoxin + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Pisum sativum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 2 reduced ferredoxin + NADP+
-
 Pisum sativum 2 oxidized ferredoxin + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.18.1.2 More FNR-membrane recruitment motif, FNR-MRM, forms a polyproline helix that interacts with a proline recognition domain on FNR. FNR displays a pH-dependent high affinity to the MRM, loop residues contribute to multiple interactions with both FNR subunits Pisum sativum

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin:NADPH oxidoreductase
-
 Pisum sativum
1.18.1.2 FNR
-
 Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.1.2 6 8 assay at Pisum sativum

General Information

EC Number General Information Comment Organism
1.18.1.2 additional information FNR interacts with Tic62, a member of the Tic complex, i.e. translocon at the inner envelope of chloroplasts, involved in redox-regulation of protein import into chloroplasts. Tic62 represents a major FNR interaction protein partner at the thylakoids, and binding to Tic62 clearly increases the stability of FNR. The specific interaction with FNR is mediated by a conserved sequence motif rich in proline and serine residues, located in the C terminus of Tic62 Pisum sativum