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Literature summary extracted from

  • Luthra, P.M.; Singh, S.
    Identification and optimization of tyrosine hydroxylase activity in Mucuna pruriens DC. var. utilis (2010), Planta, 231, 1361-1369.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.2 0.0041
-
 tetrahydrobiopterin pH 7.0, 37°C Mucuna pruriens var. utilis
1.14.16.2 0.8086
-
 L-tyrosine pH 6.0, 37°C Mucuna pruriens var. utilis
1.14.16.2 1.1
-
 tyramine pH 7.0, 37°C Mucuna pruriens var. utilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.2 Fe2+ 2 mM Mucuna pruriens var. utilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.2 55000
-
 x * 55000, SDS-PAGE, Western blot analysis Mucuna pruriens var. utilis

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Mucuna pruriens var. utilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.2 centrifugation Mucuna pruriens var. utilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.2 leaf
-
 Mucuna pruriens var. utilis
-
1.14.16.2 pod
-
 Mucuna pruriens var. utilis
-
1.14.16.2 root
-
 Mucuna pruriens var. utilis
-
1.14.16.2 seed immature cell Mucuna pruriens var. utilis
-
1.14.16.2 stem
-
 Mucuna pruriens var. utilis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.2 101
-
 L-tyramine, crude extract of mature leaves, pH 6.0, 37°C Mucuna pruriens var. utilis
1.14.16.2 113
-
 L-tyrosine, crude extract of mature leaves, pH 6.0, 37°C Mucuna pruriens var. utilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2
-
 Mucuna pruriens var. utilis 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O
-
 ?
1.14.16.2 tyramine + tetrahydrobiopterin + O2
-
 Mucuna pruriens var. utilis dopamine + 4a-hydroxytetrahydrobiopterin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.16.2 ? x * 55000, SDS-PAGE, Western blot analysis Mucuna pruriens var. utilis

Synonyms

EC Number Synonyms Comment Organism
1.14.16.2 tyrosine 3-monooxygenase
-
 Mucuna pruriens var. utilis
1.14.16.2 tyrosine hydroxylase
-
 Mucuna pruriens var. utilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.16.2 37
-
-
 Mucuna pruriens var. utilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.16.2 20 45 60 min Mucuna pruriens var. utilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.16.2 6
-
 broad optimum pH 4.0-7.5 Mucuna pruriens var. utilis