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Literature summary extracted from

  • Hoefnagel, M.; Rich, P.R.; Zhang, Q.; Wiskich, J.T.
    Substrate kinetics of the plant mitochondrial alternative oxidase and the effects of pyruvate (1997), Plant Physiol., 115, 1145-1153.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.11 EDT-20 the addition of 0.025% (v/v) EDT-20 in the assay increases the activity 3-4fold Glycine max
1.10.3.11 EDT-20 the addition of 0.025% (v/v) EDT-20 in the assay increases the activity 3-4fold Arum italicum
1.10.3.11 pyruvate potent activator, its presence is essential for maximum activity, pyruvate increases the activity of AOX but does not increase its affinity for its substrate Glycine max
1.10.3.11 pyruvate potent activator, its presence is essential for maximum activity, pyruvate increases the activity of AOX but does not increase its affinity for its substrate Arum italicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.11 n-octyl gallate the enzyme is fully sensitive to 0.001 mM octyl gallate Arum italicum
1.10.3.11 n-octyl gallate the enzyme is fully sensitive to 0.001 mM octyl gallate Glycine max
1.10.3.11 n-propyl gallate
-
 Arum italicum
1.10.3.11 n-propyl gallate
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.11 0.1153
-
 ubiquinol-1 in the presence of EDT-20 (0.025%) and pyruvate, 0.3 M succinate, 25 mM TES, 10 mM potassium phosphate, and 2 mM magnesium sulfate, pH 7.0, at 25°C Glycine max
1.10.3.11 0.331
-
 ubiquinol-1 in the presence of EDT-20 (0.025%) and pyruvate, 0.3 M succinate, 25 mM TES, 10 mM potassium phosphate, and 2 mM magnesium sulfate, pH 7.0, at 25°C Arum italicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.11 mitochondrial membrane inner mitochondrial membrane Glycine max 31966
-
1.10.3.11 mitochondrial membrane inner mitochondrial membrane Arum italicum 31966
-

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.11 Arum italicum
-
-
-
1.10.3.11 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.11 DEAE-Sepharose column chromatography and Q-Sepharose column chromatography Arum italicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.11 cotyledon
-
 Glycine max
-
1.10.3.11 spadix
-
 Arum italicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.11 decylubiquinol + O2
-
 Glycine max decylubiquinone + H2O
-
 ?
1.10.3.11 decylubiquinol + O2
-
 Arum italicum decylubiquinone + H2O
-
 ?
1.10.3.11 duroquinol + O2
-
 Glycine max duroquinone + H2O
-
 ?
1.10.3.11 duroquinol + O2
-
 Arum italicum duroquinone + H2O
-
 ?
1.10.3.11 ubiquinol-1 + O2 most suitable substrate Glycine max ubiquinone-1 + H2O
-
 ?
1.10.3.11 ubiquinol-1 + O2 most suitable substrate Arum italicum ubiquinone-1 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.11 alternative oxidase
-
 Glycine max
1.10.3.11 alternative oxidase
-
 Arum italicum
1.10.3.11 AOX
-
 Glycine max
1.10.3.11 AOX
-
 Arum italicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.11 7 7.5
-
 Glycine max
1.10.3.11 7 7.5
-
 Arum italicum