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Literature summary extracted from

  • Bross, C.D.; Corea, O.R.; Kaldis, A.; Menassa, R.; Bernards, M.A.; Kohalmi, S.E.
    Complementation of the pha2 yeast mutant suggests functional differences for arogenate dehydratases from Arabidopsis thaliana (2011), Plant Physiol. Biochem., 49, 882-890.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.91 AtADT1, DNA and amino acid sequence determination and analysis, sequence comparison, complementation of the Saccharomyces cerevisiae pha2 mutant strain YNL316c, which lacks PDT activity and cannot grow in the absence of exogenous Phe by the six ADTs from Arabidopsis thaliana: AtADT2 readily recovers the pha2 phenotype after about 6 days growth at 30°C, while AtADT1 requires about 13 days to show visible growth. By contrast, AtADT6, with lowest PDT activity, and AtADT3-5, with no PDT activity, are unable to recover the phenotype, overview Arabidopsis thaliana
4.2.1.91 AtADT2, DNA and amino acid sequence determination and analysis, sequence comparison, complementation of the Saccharomyces cerevisiae pha2 mutant strain YNL316c, which lacks PDT activity and cannot grow in the absence of exogenous Phe by the six ADTs from Arabidopsis thaliana: AtADT2 readily recovers the pha2 phenotype after about 6 days growth at 30°C, while AtADT1 requires about 13 days to show visible growth. By contrast, AtADT6, with lowest PDT activity, and AtADT3-5, with no PDT activity, are unable to recover the phenotype, overview Arabidopsis thaliana
4.2.1.91 AtADT3, DNA and amino acid sequence determination and analysis, sequence comparison, complementation of the Saccharomyces cerevisiae pha2 mutant strain YNL316c, which lacks PDT activity and cannot grow in the absence of exogenous Phe by the six ADTs from Arabidopsis thaliana: AtADT2 readily recovers the pha2 phenotype after about 6 days growth at 30°C, while AtADT1 requires about 13 days to show visible growth. By contrast, AtADT6, with lowest PDT activity, and AtADT3-5, with no PDT activity, are unable to recover the phenotype, overview Arabidopsis thaliana
4.2.1.91 AtADT4, DNA and amino acid sequence determination and analysis, sequence comparison, complementation of the Saccharomyces cerevisiae pha2 mutant strain YNL316c, which lacks PDT activity and cannot grow in the absence of exogenous Phe by the six ADTs from Arabidopsis thaliana: AtADT2 readily recovers the pha2 phenotype after about 6 days growth at 30°C, while AtADT1 requires about 13 days to show visible growth. By contrast, AtADT6, with lowest PDT activity, and AtADT3-5, with no PDT activity, are unable to recover the phenotype, overview Arabidopsis thaliana
4.2.1.91 AtADT5, DNA and amino acid sequence determination and analysis, sequence comparison, complementation of the Saccharomyces cerevisiae pha2 mutant strain YNL316c, which lacks PDT activity and cannot grow in the absence of exogenous Phe by the six ADTs from Arabidopsis thaliana: AtADT2 readily recovers the pha2 phenotype after about 6 days growth at 30°C, while AtADT1 requires about 13 days to show visible growth. By contrast, AtADT6, with lowest PDT activity, and AtADT3-5, with no PDT activity, are unable to recover the phenotype, overview Arabidopsis thaliana
4.2.1.91 AtADT6, DNA and amino acid sequence determination and analysis, sequence comparison, complementation of the Saccharomyces cerevisiae pha2 mutant strain YNL316c, which lacks PDT activity and cannot grow in the absence of exogenous Phe by the six ADTs from Arabidopsis thaliana: AtADT2 readily recovers the pha2 phenotype after about 6 days growth at 30°C, while AtADT1 requires about 13 days to show visible growth. By contrast, AtADT6, with lowest PDT activity, and AtADT3-5, with no PDT activity, are unable to recover the phenotype, overview Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.51 prephenate Arabidopsis thaliana
-
 phenylpyruvate + H2O + CO2
-
 ?
4.2.1.91 L-arogenate Arabidopsis thaliana
-
 L-phenylalanine + H2O + CO2
-
 ?
4.2.1.91 additional information Arabidopsis thaliana only AtADT1 and AtADT2, but not the other four ADTs from Arabidopsis thaliana, have functional PDT prephenate dehydratase, EC 4.2.1.51, activity in vivo ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.51 Arabidopsis thaliana
-
-
-
4.2.1.91 Arabidopsis thaliana O22241 isozyme AtADT4; isozymes AtADT1-AtADT6
-
4.2.1.91 Arabidopsis thaliana Q9FNJ8 isozyme AtADT5; isozymes AtADT1-AtADT6
-
4.2.1.91 Arabidopsis thaliana Q9SA96 isozyme AtADT1; isozymes AtADT1-AtADT6
-
4.2.1.91 Arabidopsis thaliana Q9SGD6 isozyme AtADT6; isozymes AtADT1-AtADT6
-
4.2.1.91 Arabidopsis thaliana Q9SSE7 isozyme AtADT2; isozymes AtADT1-AtADT6
-
4.2.1.91 Arabidopsis thaliana Q9ZUY3 isozyme AtADT3; isozymes AtADT1-AtADT6
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.51 prephenate
-
 Arabidopsis thaliana phenylpyruvate + H2O + CO2
-
 ?
4.2.1.91 L-arogenate
-
 Arabidopsis thaliana L-phenylalanine + H2O + CO2
-
 ?
4.2.1.91 additional information only AtADT1 and AtADT2, but not the other four ADTs from Arabidopsis thaliana, have functional PDT prephenate dehydratase, EC 4.2.1.51, activity in vivo Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.51 ADT1 the arogenate dehydratase isoform has limited activity with prephenate Arabidopsis thaliana
4.2.1.51 ADT2 the arogenate dehydratase isoform has limited activity with prephenate Arabidopsis thaliana
4.2.1.51 ADT6 the arogenate dehydratase isoform has limited activity with prephenate Arabidopsis thaliana
4.2.1.51 PDT
-
 Arabidopsis thaliana
4.2.1.91 AdT
-
 Arabidopsis thaliana
4.2.1.91 AtADT1
-
 Arabidopsis thaliana
4.2.1.91 AtADT2
-
 Arabidopsis thaliana
4.2.1.91 AtADT3
-
 Arabidopsis thaliana
4.2.1.91 AtADT4
-
 Arabidopsis thaliana
4.2.1.91 AtADT5
-
 Arabidopsis thaliana
4.2.1.91 AtADT6
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
4.2.1.91 evolution plant ADTs and prephenate dehydratases, EC 4.2.1.51, share many common features allowing them to act as dehydratase/decarboxylases, but group independently conferring distinct substrate specificities, sequence comparisons, overview Arabidopsis thaliana
4.2.1.91 metabolism The final steps of phenylalanine biosynthesis in bacteria, fungi and plants can occur via phenylpyruvate or arogenate intermediates. These routes are determined by the presence of prephenate dehydratase, EC 4.2.1.51, which forms phenylpyruvate from prephenate, or arogenate dehydratase, which forms phenylalanine directly from arogenate Arabidopsis thaliana