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Literature summary extracted from

  • Zhuang, Y.; Ren, G.; He, C.; Li, X.; Meng, Q.; Zhu, C.; Wang, R.; Zhang, J.
    Cloning and characterization of a maize cDNA encoding glutamate decarboxylase (2010), Plant Mol. Biol. Rep., 28, 620-626.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.15 expressed in Escherichia coli BL21(DE3) cells Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.15 56200
-
 x * 56200, calculated from amino acid sequence Zea mays
4.1.1.15 61000
-
 x * 61000, His-tagged enzyme, SDS-PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.15 Zea mays
-
 inbred line DH4866
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.15 His binding resin column chromatography Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.15 corn ear the enzyme is most abundantly expressed in the corn ears Zea mays
-
4.1.1.15 leaf
-
 Zea mays
-
4.1.1.15 root
-
 Zea mays
-
4.1.1.15 stem
-
 Zea mays
-
4.1.1.15 tassel
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.15 L-glutamate
-
 Zea mays 4-aminobutanoate + CO2
-
 ir

Subunits

EC Number Subunits Comment Organism
4.1.1.15 ? x * 56200, calculated from amino acid sequence Zea mays
4.1.1.15 ? x * 61000, His-tagged enzyme, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
4.1.1.15 GAD1
-
 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.15 5.8
-
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.15 Calmodulin
-
 Zea mays
4.1.1.15 pyridoxal 5'-phosphate
-
 Zea mays

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.15 Zea mays calculated from amino acid sequence
-
 5.4

Expression

EC Number Organism Comment Expression
4.1.1.15 Zea mays GAD1 gene is upregulated and GAD activity is increased in the leaves and roots after treatment with 0.1 mM ABA, 0.1 mM methyl jasmonate, 0.8% (w/v) NaCl, 20% (w/v) polyethylene glycol, or cold stress up

General Information

EC Number General Information Comment Organism
4.1.1.15 physiological function GAD1 plays an important role in responses to abiotic factors and hormone treatments Zea mays