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Literature summary extracted from

  • Hall, D.E.; Robert, J.A.; Keeling, C.I.; Domanski, D.; Quesada, A.L.; Jancsik, S.; Kuzyk, M.A.; Hamberger, B.; Borchers, C.H.; Bohlmann, J.
    An integrated genomic, proteomic and biochemical analysis of (+)-3-carene biosynthesis in Sitka spruce (Picea sitchensis) genotypes that are resistant or susceptible to white pine weevil (2011), Plant J., 65, 936-948.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.107 methyl jasmonate protein is detected at significantly higher levels in susceptible trees compared to resistant trees at day 7 after methyl jasmonate treatment Picea sitchensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.107
-
 Picea sitchensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.107 additional information
-
 additional information isoform 3car3 has an extremely low activity Picea sitchensis
4.2.3.107 0.00688
-
 geranyl diphosphate pH 7.4, 30°C, proteins from both susceptible and resistant plants have similar kinetic parameters Picea sitchensis

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.107 Picea sitchensis F1CKI6 isoform TPS-3car1, expressed both in trees susceptible and resistant to white pine weevil
-
4.2.3.107 Picea sitchensis F1CKI8 isoform TPS-3car2, only expressed in trees resistant to white pine weevil
-
4.2.3.107 Picea sitchensis F1CKI9 isoform TPS-3car3, only expressed in trees susceptilble to white pine weevil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.107
-
 Picea sitchensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.107 stem
-
 Picea sitchensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.107 geranyl diphosphate
-
 Picea sitchensis (+)-car-3-ene + diphosphate major product, plus some terpinolene ?
4.2.3.107 additional information poor substrate: neryl diphosphate. No substrates: farnesyl diphosphate, geranylgeranyl diphosphate Picea sitchensis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.107 TPS-3car1
-
 Picea sitchensis
4.2.3.107 TPS-3car2
-
 Picea sitchensis
4.2.3.107 TPS-3car3
-
 Picea sitchensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.107 2.21
-
 geranyl diphosphate pH 7.4, 30°C, proteins from both susceptible and resistant plants have similar kinetic parameters Picea sitchensis

Expression

EC Number Organism Comment Expression
4.2.3.107 Picea sitchensis transcript levels are increased following methyl jasmonate treatment, with transcripts detected at significantly higher abundance in resistant trees compared to susceptible trees up

General Information

EC Number General Information Comment Organism
4.2.3.107 physiological function (+)-3-carene is associated with resistance of Sitka spruce to white pine weevil, Pissodes strobi. Variation of the (+)-3-carene phenotype is controlled by copy number variation of TPS-3car genes, variation of gene and protein expression, and variation in catalytic efficiencies Picea sitchensis