Literature summary extracted from

Kamiyoshihara, Y.; Iwata, M.; Fukaya, T.; Tatsuki, M.; Mori, H.
Turnover of LeACS2, a wound-inducible 1-aminocyclopropane-1-carboxylic acid synthase in tomato, is regulated by phosphorylation/dephosphorylation (2010), Plant J., 64, 140-150.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.4.1.14	expression in Escherichia coli	Solanum lycopersicum

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.4.1.14	Solanum lycopersicum	P18485	isoform ACS2	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
4.4.1.14	phosphoprotein	phosphorylation/dephosphorylation of ACS2 regulates its turnover upstream of the ubiquitin-26S-proteasome degradation pathway. ACS2 is stabilized by phosphorylation and degraded after dephosphorylation. The amount of ACS2 affected by the protein kinase/phosphatase inhibitors significantly influences cellular ACS activity, 1-aminocyclopropane-1-carboxylic acid content, and ethylene production levels in tomato fruit tissue. Calcium-dependent protein kinase CDPK2, is one of the protein kinases that are able to phosphorylate ACS2 at residue S460. ACS2 is immediately phosphorylated after translation by CDPK and mitogen-activated protein kinase at different sites in response to wound signaling and almost all functional ACS2 molecules are phosphorylated in the cell. Phosphorylation at both sites is required for ACS2 stability	Solanum lycopersicum

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
4.4.1.14	fruit	-	Solanum lycopersicum	-

Synonyms

EC Number	Synonyms	Comment	Organism
4.4.1.14	ACS2	-	Solanum lycopersicum

General Information

EC Number	General Information	Comment	Organism
4.4.1.14	metabolism	phosphorylation/dephosphorylation of ACS2 regulates its turnover upstream of the ubiquitin-26S-proteasome degradation pathway. ACS2 is stabilized by phosphorylation and degraded after dephosphorylation. The amount of ACS2 affected by the protein kinase/phosphatase inhibitors significantly influences cellular ACS activity, 1-aminocyclopropane-1-carboxylic acid content, and ethylene production levels in tomato fruit tissue. Calcium-dependent protein kinase CDPK2, is one of the protein kinases that are able to phosphorylate ACS2 at residue S460. ACS2 is immediately phosphorylated after translation by CDPK and mitogen-activated protein kinase at different sites in response to wound signaling and almost all functional ACS2 molecules are phosphorylated in the cell. Phosphorylation at both sites is required for ACS2 stability	Solanum lycopersicum

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Release 2023.1 (January 2023)