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Literature summary extracted from

  • Lee, E.J.; Facchini, P.
    Norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family (2010), Plant Cell, 22, 3489-3503.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.78 NCS2-green fluorescent protein fusion introduced by microprojectile bombardment into opium poppy cells initially localizes to the endoplasmic reticulum but subsequently sorts to the vacuole Papaver somniferum

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.78 additional information virus-induced gene silencing of NCS in opium poppy profoundly reduces alkaloid levels compared to controls, a mixed culture of Agrobacterium tumefaciens harboring either pTRV2-PsNCS or pTRV1 is infiltrated into opium poppy seedlings tow weeks after germination. Levels of the five major alkaloids in opium poppy latex, i.e. morphine, codeine, thebaine, noscapine, and papaveine, are reduced by 75-82% in plants infiltrated with Agrobacterium tumefaciens harboring pTRV2-PsNCS Papaver somniferum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.78 additional information immunoprecipitation of NCS from total protein extracts of Thalictrum flavum cells results in a nearly complete attenuation of NCS activity Thalictrum flavum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.78 additional information immunolocalization study of NCS, overview Papaver somniferum
-
-
4.2.1.78 soluble
-
Thalictrum flavum
-
-
4.2.1.78 vacuole NCS2 has an N-terminal signal peptide and sorts to the vacuole Papaver somniferum 5773
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.78 4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol Papaver somniferum 4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine (S)-norcoclaurine + H2O
-
?
4.2.1.78 4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol Coptis japonica 4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine (S)-norcoclaurine + H2O
-
?
4.2.1.78 4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol Thalictrum flavum 4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine (S)-norcoclaurine + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.78 Coptis japonica
-
-
-
4.2.1.78 Papaver somniferum
-
isozymes NCS1 and NCS2
-
4.2.1.78 Thalictrum flavum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.78 carpel low level Papaver somniferum
-
4.2.1.78 cell culture
-
Thalictrum flavum
-
4.2.1.78 flower bud very low expression level Thalictrum flavum
-
4.2.1.78 leaf low level Papaver somniferum
-
4.2.1.78 additional information not in leaves Thalictrum flavum
-
4.2.1.78 phloem NCS2 shows cell type-specific occurrence in phloem sieve elements, colocalization with all other known benzylisoquinoline alkaloid biosynthetic enzymes Papaver somniferum
-
4.2.1.78 rhizome
-
Thalictrum flavum
-
4.2.1.78 root
-
Thalictrum flavum
-
4.2.1.78 root abundant Papaver somniferum
-
4.2.1.78 stem abundant Papaver somniferum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.78 4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol 4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine Papaver somniferum (S)-norcoclaurine + H2O
-
?
4.2.1.78 4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol 4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine Coptis japonica (S)-norcoclaurine + H2O
-
?
4.2.1.78 4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol 4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine Thalictrum flavum (S)-norcoclaurine + H2O
-
?
4.2.1.78 additional information isozyme NCS1 of Coptis japonica does not catalyze the formation of (S)-norcoclaurine from dopamine and 4-hydroxyphenylacetaldehyde Coptis japonica ?
-
?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.78 More norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family Papaver somniferum
4.2.1.78 More norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family Coptis japonica
4.2.1.78 More norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family Thalictrum flavum
4.2.1.78 NCS
-
Papaver somniferum
4.2.1.78 NCS
-
Coptis japonica
4.2.1.78 NCS
-
Thalictrum flavum
4.2.1.78 NCS1
-
Papaver somniferum
4.2.1.78 NCS1
-
Coptis japonica
4.2.1.78 NCS2
-
Papaver somniferum
4.2.1.78 norcoclaurine synthase
-
Papaver somniferum
4.2.1.78 norcoclaurine synthase
-
Coptis japonica
4.2.1.78 norcoclaurine synthase
-
Thalictrum flavum
4.2.1.78 PR10A
-
Coptis japonica

Expression

EC Number Organism Comment Expression
4.2.1.78 Papaver somniferum elicitor-induced cell cultures show increasing expression of NCS after 2 to 100 hours up

General Information

EC Number General Information Comment Organism
4.2.1.78 metabolism norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview Papaver somniferum
4.2.1.78 metabolism norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview Coptis japonica
4.2.1.78 metabolism norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview Thalictrum flavum
4.2.1.78 additional information amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview Papaver somniferum
4.2.1.78 additional information amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview Coptis japonica
4.2.1.78 additional information amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview Thalictrum flavum