EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.78 | NCS2-green fluorescent protein fusion introduced by microprojectile bombardment into opium poppy cells initially localizes to the endoplasmic reticulum but subsequently sorts to the vacuole | Papaver somniferum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.78 | additional information | virus-induced gene silencing of NCS in opium poppy profoundly reduces alkaloid levels compared to controls, a mixed culture of Agrobacterium tumefaciens harboring either pTRV2-PsNCS or pTRV1 is infiltrated into opium poppy seedlings tow weeks after germination. Levels of the five major alkaloids in opium poppy latex, i.e. morphine, codeine, thebaine, noscapine, and papaveine, are reduced by 75-82% in plants infiltrated with Agrobacterium tumefaciens harboring pTRV2-PsNCS | Papaver somniferum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.78 | additional information | immunoprecipitation of NCS from total protein extracts of Thalictrum flavum cells results in a nearly complete attenuation of NCS activity | Thalictrum flavum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.1.78 | additional information | immunolocalization study of NCS, overview | Papaver somniferum | - |
- |
4.2.1.78 | soluble | - |
Thalictrum flavum | - |
- |
4.2.1.78 | vacuole | NCS2 has an N-terminal signal peptide and sorts to the vacuole | Papaver somniferum | 5773 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.78 | 4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol | Papaver somniferum | 4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine | (S)-norcoclaurine + H2O | - |
? | |
4.2.1.78 | 4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol | Coptis japonica | 4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine | (S)-norcoclaurine + H2O | - |
? | |
4.2.1.78 | 4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol | Thalictrum flavum | 4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine | (S)-norcoclaurine + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.78 | Coptis japonica | - |
- |
- |
4.2.1.78 | Papaver somniferum | - |
isozymes NCS1 and NCS2 | - |
4.2.1.78 | Thalictrum flavum | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.1.78 | carpel | low level | Papaver somniferum | - |
4.2.1.78 | cell culture | - |
Thalictrum flavum | - |
4.2.1.78 | flower bud | very low expression level | Thalictrum flavum | - |
4.2.1.78 | leaf | low level | Papaver somniferum | - |
4.2.1.78 | additional information | not in leaves | Thalictrum flavum | - |
4.2.1.78 | phloem | NCS2 shows cell type-specific occurrence in phloem sieve elements, colocalization with all other known benzylisoquinoline alkaloid biosynthetic enzymes | Papaver somniferum | - |
4.2.1.78 | rhizome | - |
Thalictrum flavum | - |
4.2.1.78 | root | - |
Thalictrum flavum | - |
4.2.1.78 | root | abundant | Papaver somniferum | - |
4.2.1.78 | stem | abundant | Papaver somniferum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.78 | 4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol | 4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine | Papaver somniferum | (S)-norcoclaurine + H2O | - |
? | |
4.2.1.78 | 4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol | 4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine | Coptis japonica | (S)-norcoclaurine + H2O | - |
? | |
4.2.1.78 | 4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol | 4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine | Thalictrum flavum | (S)-norcoclaurine + H2O | - |
? | |
4.2.1.78 | additional information | isozyme NCS1 of Coptis japonica does not catalyze the formation of (S)-norcoclaurine from dopamine and 4-hydroxyphenylacetaldehyde | Coptis japonica | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.78 | More | norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family | Papaver somniferum |
4.2.1.78 | More | norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family | Coptis japonica |
4.2.1.78 | More | norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family | Thalictrum flavum |
4.2.1.78 | NCS | - |
Papaver somniferum |
4.2.1.78 | NCS | - |
Coptis japonica |
4.2.1.78 | NCS | - |
Thalictrum flavum |
4.2.1.78 | NCS1 | - |
Papaver somniferum |
4.2.1.78 | NCS1 | - |
Coptis japonica |
4.2.1.78 | NCS2 | - |
Papaver somniferum |
4.2.1.78 | norcoclaurine synthase | - |
Papaver somniferum |
4.2.1.78 | norcoclaurine synthase | - |
Coptis japonica |
4.2.1.78 | norcoclaurine synthase | - |
Thalictrum flavum |
4.2.1.78 | PR10A | - |
Coptis japonica |
EC Number | Organism | Comment | Expression |
---|---|---|---|
4.2.1.78 | Papaver somniferum | elicitor-induced cell cultures show increasing expression of NCS after 2 to 100 hours | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.78 | metabolism | norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview | Papaver somniferum |
4.2.1.78 | metabolism | norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview | Coptis japonica |
4.2.1.78 | metabolism | norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview | Thalictrum flavum |
4.2.1.78 | additional information | amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview | Papaver somniferum |
4.2.1.78 | additional information | amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview | Coptis japonica |
4.2.1.78 | additional information | amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview | Thalictrum flavum |