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Literature summary extracted from

  • Yin, R.; Frey, M.; Gierl, A.; Glawischnig, E.
    Plants contain two distinct classes of functional tryptophan synthase beta proteins (2010), Phytochemistry, 71, 1667-1672.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.20 TSB phylogenetic analysis and tree, functional expression of isozyme AtTSBtype2 in Escherichia coli Arabidopsis thaliana
4.2.1.20 TSB phylogenetic analysis and tree, functional expression of isozyme ZmTSBtype2 in Escherichia coli Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.20 additional information construction of Attsbtype2 T-DNA insertion mutants,SALK lines 011904, 124293, 082810, and 162268, that show no obvious deviation from the wild-type Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.20 0.000074
-
 indole recombinant isozyme AtTSBtype2, pH 8.2, 30°C Arabidopsis thaliana
4.2.1.20 0.007
-
 indole recombinant isozyme TSB type 2, pH 8.2, 30°C Zea mays
4.2.1.20 6.2
-
 L-serine recombinant isozyme TSB type 2, pH 8.2, 30°C Zea mays
4.2.1.20 35
-
 L-serine recombinant isozyme AtTSBtype2, pH 8.2, 30°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Arabidopsis thaliana Q9FFW8 isozymes AtTSB1 and AtTSBtype2
-
4.2.1.20 Arabidopsis thaliana Col-0 Q9FFW8 isozymes AtTSB1 and AtTSBtype2
-
4.2.1.20 Zea mays
-
 isozyme TSB type 2
-
4.2.1.20 Zea mays B73
-
 isozyme TSB type 2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.20 recombinant isozyme AtTSBtype2 from Escherichia coli by nickel affinity chromatography Arabidopsis thaliana
4.2.1.20 recombinant isozyme ZmTSBtype2 from Escherichia coli by nickel affinity chromatography Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.20 additional information maize isozyme TSB type 2 is transcribed in various tissues Zea mays
-
4.2.1.20 additional information while AtTSB1 is the predominantly expressed isoform in vegetative tissues, AtTSB1 and AtTSBtype2 reach similar transcript levels in seeds, tissue-specific expression pattern, overview Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 L-serine + indole
-
 Zea mays L-tryptophan + H2O
-
 ?
4.2.1.20 L-serine + indole
-
 Arabidopsis thaliana L-tryptophan + H2O
-
 ?
4.2.1.20 L-serine + indole
-
 Arabidopsis thaliana Col-0 L-tryptophan + H2O
-
 ?
4.2.1.20 L-serine + indole
-
 Zea mays B73 L-tryptophan + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.20 tryptophan synthase beta
-
 Zea mays
4.2.1.20 tryptophan synthase beta
-
 Arabidopsis thaliana
4.2.1.20 TSB
-
 Zea mays
4.2.1.20 TSB
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.20 30
-
 assay at Zea mays
4.2.1.20 30
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.20 0.007
-
 indole recombinant isozyme AtTSBtype2, pH 8.2, 30°C Arabidopsis thaliana
4.2.1.20 0.0102
-
 indole recombinant isozyme TSB type 2, pH 8.2, 30°C Zea mays
4.2.1.20 0.0155
-
 L-serine recombinant isozyme AtTSBtype2, pH 8.2, 30°C Arabidopsis thaliana
4.2.1.20 0.0223
-
 L-serine recombinant isozyme TSB type 2, pH 8.2, 30°C Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.20 8.2
-
 assay at Zea mays
4.2.1.20 8.2
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.20 pyridoxal 5'-phosphate
-
 Zea mays
4.2.1.20 pyridoxal 5'-phosphate
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
4.2.1.20 physiological function AtTSBtype2 function is not essential under standard growth conditions Arabidopsis thaliana