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Literature summary extracted from

  • Smith, J.; Ververidis, P.; John, P.
    Characterization of the ethylene-forming enzyme partially purified from melon (1992), Phytochemistry, 31, 1485-1494.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.4 2,4,6-Trinitrobenzenesulfonate 5 mM, 0.7% residual activity Cucumis melo
1.14.17.4 2,4-dinitrophenol 1 mM, 40% residual activity Cucumis melo
1.14.17.4 4-Chloromercuriphenylsulfonate 1 mM, 1.7% residual activity Cucumis melo
1.14.17.4 EDTA 5 mM, 2.1% residual activity Cucumis melo
1.14.17.4 N-ethylmaleimide 1 mM, 10% residual activity Cucumis melo
1.14.17.4 n-propyl gallate 3 mM, 1.3% residual activity Cucumis melo
1.14.17.4 Salicylhydroxamic acid 1 mM, 15% residual activity Cucumis melo

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.17.4 0.06
-
 1-aminocyclopropane-1-carboxylate pH 7.5, 30°C Cucumis melo

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.4 Fe2+
-
 Cucumis melo

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.17.4 41000
-
 x * 41000, SDS-PAGE Cucumis melo

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.4 Cucumis melo
-
 L. var Galia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.4 partial Cucumis melo

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2
-
 Cucumis melo ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 additional information 2-oxoglutarate is not required for activity Cucumis melo ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.17.4 ? x * 41000, SDS-PAGE Cucumis melo

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.17.4 7.5
-
-
 Cucumis melo

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.4 ascorbate absolutely required Cucumis melo
1.14.17.4 isoascorbate may substitute for ascorbate, 75% of the efficiency of ascorbate Cucumis melo