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Literature summary extracted from

  • Shull, N.P.; Spinelli, S.L.; Phizicky, E.M.
    A highly specific phosphatase that acts on ADP-ribose 1''-phosphate, a metabolite of tRNA splicing in Saccharomyces cerevisiae (2005), Nucleic Acids Res., 33, 650-660.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.84 2'-CMP
-
 Saccharomyces cerevisiae
3.1.3.84 3'-AMP
-
 Saccharomyces cerevisiae
3.1.3.84 ADP
-
 Saccharomyces cerevisiae
3.1.3.84 ADP-ribose strong inhibition Saccharomyces cerevisiae
3.1.3.84 AppA
-
 Saccharomyces cerevisiae
3.1.3.84 additional information Poa1p is not inhibited by 5'3'-ADP , 5'-AMP, 2'-AMP, 2'-GMP, 3'-GMP, 3'-CMP, D-ribose-1-phosphate, D-ribose-5-phosphate, D-glucose-1-phosphate, D-fructose-6-phosphate, and glyceraldehyde-3-phosphate Saccharomyces cerevisiae
3.1.3.84 NAD+
-
 Saccharomyces cerevisiae
3.1.3.84 NADP+
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.84 0.0028
-
 ADP-ribose 1''-phosphate in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.84 27000
-
 estimated from amino acid sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.84 ADP-ribose 1''-phosphate + H2O Saccharomyces cerevisiae Poa1p is highly specific for ADP-ribose 1''-phosphate, Poa1p is responsible for about 90% of ADP-ribose 1''-phosphate processing activity in yeast extracts ADP-ribose + phosphate
-
 ?
3.1.3.84 ADP-ribose 1''-phosphate + H2O Saccharomyces cerevisiae NSY32 Poa1p is highly specific for ADP-ribose 1''-phosphate, Poa1p is responsible for about 90% of ADP-ribose 1''-phosphate processing activity in yeast extracts ADP-ribose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.84 Saccharomyces cerevisiae P38218
-
-
3.1.3.84 Saccharomyces cerevisiae NSY32 P38218
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.84 Talon resin column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.84 ADP-ribose 1''-phosphate + H2O Poa1p is highly specific for ADP-ribose 1''-phosphate, Poa1p is responsible for about 90% of ADP-ribose 1''-phosphate processing activity in yeast extracts Saccharomyces cerevisiae ADP-ribose + phosphate
-
 ?
3.1.3.84 ADP-ribose 1''-phosphate + H2O Poa1p is highly specific for ADP-ribose 1''-phosphate Saccharomyces cerevisiae ADP-ribose + phosphate
-
 ?
3.1.3.84 ADP-ribose 1''-phosphate + H2O Poa1p is highly specific for ADP-ribose 1''-phosphate, Poa1p is responsible for about 90% of ADP-ribose 1''-phosphate processing activity in yeast extracts Saccharomyces cerevisiae NSY32 ADP-ribose + phosphate
-
 ?
3.1.3.84 ADP-ribose 1''-phosphate + H2O Poa1p is highly specific for ADP-ribose 1''-phosphate Saccharomyces cerevisiae NSY32 ADP-ribose + phosphate
-
 ?
3.1.3.84 additional information Poa1p does not recognize pAp as substrate Saccharomyces cerevisiae ?
-
 ?
3.1.3.84 additional information Poa1p does not recognize pAp as substrate Saccharomyces cerevisiae NSY32 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.84 Appr1p phosphatase
-
 Saccharomyces cerevisiae
3.1.3.84 ORF YBR022w
-
 Saccharomyces cerevisiae
3.1.3.84 Poa1p
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.84 0.028
-
 ADP-ribose 1''-phosphate in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.84 6
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.84 5 7
-
 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.84 0.03
-
 ADP-ribose in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae
3.1.3.84 1
-
 AppA in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae
3.1.3.84 1.5
-
 NAD+ in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae
3.1.3.84 2
-
 NADP+ in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae
3.1.3.84 10
-
 ADP Ki above 10 mM, in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae
3.1.3.84 10
-
 3'-AMP Ki above 10 mM, in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae
3.1.3.84 10
-
 2'-CMP Ki above 10 mM, in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.84 10
-
 ADP-ribose 1''-phosphate in 20 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 2.5 mM spermidine, 100 mM dithiothreitol, 0.4% (v/v) Triton X-100, at 30°C Saccharomyces cerevisiae