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Literature summary extracted from

  • Bogan, K.; Brenner, C.
    5'-Nucleotidases and their new roles in NAD+ and phosphate metabolism (2010), New J. Chem., 34, 845-853.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.5 2,3-bisphosphoglycerate activates cytosolic 5'-nucleotidase II Homo sapiens
3.1.3.5 ATP activates cytosolic 5'-nucleotidase II Homo sapiens
3.1.3.5 dATP activates cytosolic 5'-nucleotidase II Homo sapiens
3.1.3.5 Diadenosine tetraphosphate activates cytosolic 5'-nucleotidase II by increase binding and dimer formation, interface binding structure modeling Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.5 gene NT5E at 6q14-q21 encodes CD73 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 alpha/beta-methylene-ADP a specific CD73 inhibitor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 additional information
-
 additional information complex allosteric regulation of cytosolic 5'-nucleotidase II Homo sapiens
3.1.3.5 1.2
-
 5'-UMP 5'-nucleotidase Sdt1, pH not specified in the publication, temperature not specified in the publication Saccharomyces cerevisiae
3.1.3.5 2.3
-
 5'-CMP 5'-nucleotidase Sdt1, pH not specified in the publication, temperature not specified in the publication Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 cell surface CD73 Homo sapiens 9986
-
3.1.3.5 cytosol
-
 Homo sapiens 5829
-
3.1.3.5 extracellular
-
 Corynebacterium glutamicum
-
-
3.1.3.5 extracellular cell surface Homo sapiens
-
-
3.1.3.5 intracellular
-
 Homo sapiens 5622
-
3.1.3.5 membrane membrane-bound 5'-nucleotidases are ectoenzymes Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 71000
-
 homodimeric CD73 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.5 5'-AMP + H2O Homo sapiens
-
 adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O Homo sapiens
-
 cytidine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O Saccharomyces cerevisiae Sdt1 cytidine + phosphate
-
 ?
3.1.3.5 5'-dCMP + H2O Homo sapiens
-
 2'-deoxycytidine + phosphate
-
 ?
3.1.3.5 5'-dGMP + H2O Homo sapiens
-
 deoxyguanosine + phosphate
-
 ?
3.1.3.5 5'-dIMP + H2O Homo sapiens
-
 deoxyinosine + phosphate
-
 ?
3.1.3.5 5'-dTMP + H2O Homo sapiens
-
 deoxythymidine + phosphate
-
 ?
3.1.3.5 5'-dUMP + H2O Homo sapiens
-
 deoxyuridine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O Homo sapiens
-
 guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O Homo sapiens
-
 inosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O Saccharomyces cerevisiae Isn1 inosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O Homo sapiens
-
 uridine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O Saccharomyces cerevisiae Sdt1 uridine + phosphate
-
 ?
3.1.3.5 5'-XMP + H2O Homo sapiens
-
 xanthosine + phosphate
-
 ?
3.1.3.5 additional information Saccharomyces cerevisiae Sdt1 is highly specific for 5'-UMP and 5'-CMP, and shows negligible activity on purine nucleoside monophosphates, 3'-monophosphates or nucleoside triphosphates ?
-
 ?
3.1.3.5 additional information Homo sapiens Usha, Pho5, Sdt1 and Phm8 are phosphate starvation-induced 5'-nucleotidases with diverse substrate specificities that liberate phosphate under phosphate starvation conditions ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Corynebacterium glutamicum
-
-
-
3.1.3.5 Homo sapiens
-
 gene NT5E at 6q14-q21 encodes CD73
-
3.1.3.5 Saccharomyces cerevisiae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 B-lymphocyte CD73 is a lymphocyte surface antigen Homo sapiens
-
3.1.3.5 dendritic cell CD73 Homo sapiens
-
3.1.3.5 forestomach CD73 Homo sapiens
-
3.1.3.5 kidney CD73 Homo sapiens
-
3.1.3.5 additional information the enzyme is ubiquitously expressed among cell types. Quantitative real time-PCR for determination of CD73 distribution. CD73 is widely expressed in vertebrate tissues and is prominently expressed on B and T cells and certain types of dendritic cells. It shows variable expression among cell types within other tissues including skeletal muscle, thymus, spleen, kidney and forestomach Homo sapiens
-
3.1.3.5 skeletal muscle CD73 Homo sapiens
-
3.1.3.5 spleen CD73 Homo sapiens
-
3.1.3.5 T-lymphocyte CD73 is a lymphocyte surface antigen Homo sapiens
-
3.1.3.5 thymus CD73 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 16600
-
 substrate IMP, 5'-nucleotidase Isn1, pH not specified in the publication, temperature not specified in the publication Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 2'-chloro-2'-deoxyAMP + H2O cNII Homo sapiens 2'-chloro-2'-deoxyadenosine + phosphate
-
 ?
3.1.3.5 3'-azido-3'deoxy-dTMP + H2O cNII Homo sapiens 3'-azido-3'-deoxythymidine + phosphate
-
 ?
3.1.3.5 5'-AMP + H2O
-
 Homo sapiens adenosine + phosphate
-
 ?
3.1.3.5 5'-AMP + H2O CD73 Homo sapiens adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O
-
 Homo sapiens cytidine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O Sdt1 Saccharomyces cerevisiae cytidine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O CD73 Homo sapiens cytidine + phosphate
-
 ?
3.1.3.5 5'-dCMP + H2O
-
 Homo sapiens 2'-deoxycytidine + phosphate
-
 ?
3.1.3.5 5'-dCMP + H2O CD73 Homo sapiens 2'-deoxycytidine + phosphate
-
 ?
3.1.3.5 5'-dGMP + H2O
-
 Homo sapiens deoxyguanosine + phosphate
-
 ?
3.1.3.5 5'-dGMP + H2O cNII Homo sapiens deoxyguanosine + phosphate
-
 ?
3.1.3.5 5'-dIMP + H2O
-
 Homo sapiens deoxyinosine + phosphate
-
 ?
3.1.3.5 5'-dIMP + H2O cNII Homo sapiens deoxyinosine + phosphate
-
 ?
3.1.3.5 5'-dTMP + H2O
-
 Homo sapiens deoxythymidine + phosphate
-
 ?
3.1.3.5 5'-dTMP + H2O CD73 Homo sapiens deoxythymidine + phosphate
-
 ?
3.1.3.5 5'-dUMP + H2O
-
 Homo sapiens deoxyuridine + phosphate
-
 ?
3.1.3.5 5'-dUMP + H2O CD73 Homo sapiens deoxyuridine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Homo sapiens guanosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O cNII, CD73 Homo sapiens guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Homo sapiens inosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O Isn1 Saccharomyces cerevisiae inosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O cNII, CD73 Homo sapiens inosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O
-
 Homo sapiens uridine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O Sdt1 Saccharomyces cerevisiae uridine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O CD73 Homo sapiens uridine + phosphate
-
 ?
3.1.3.5 5'-XMP + H2O
-
 Homo sapiens xanthosine + phosphate
-
 ?
3.1.3.5 5'-XMP + H2O cNII, CD73 Homo sapiens xanthosine + phosphate
-
 ?
3.1.3.5 5-fluoro-dUMP + H2O cNII Homo sapiens 5-fluoro-uridine + phosphate
-
 ?
3.1.3.5 additional information Sdt1 is highly specific for 5'-UMP and 5'-CMP, and shows negligible activity on purine nucleoside monophosphates, 3'-monophosphates or nucleoside triphosphates Saccharomyces cerevisiae ?
-
 ?
3.1.3.5 additional information Usha, Pho5, Sdt1 and Phm8 are phosphate starvation-induced 5'-nucleotidases with diverse substrate specificities that liberate phosphate under phosphate starvation conditions Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 homodimer CD73 Homo sapiens
3.1.3.5 tetramer cytosolic 5'-nucleotidase II, cNII, is a tetramer, formed from two homodimers Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.3.5 5'-nucleotidase
-
 Corynebacterium glutamicum
3.1.3.5 CD73
-
 Homo sapiens
3.1.3.5 cNII
-
 Homo sapiens
3.1.3.5 cytosolic 5'-nucleotidase
-
 Homo sapiens
3.1.3.5 cytosolic 5'-nucleotidase II
-
 Homo sapiens
3.1.3.5 Isn1
-
 Saccharomyces cerevisiae
3.1.3.5 membrane-bound 5'-nucleotidase
-
 Homo sapiens
3.1.3.5 More cytosolic 5'-nucleotidases are members of the haloacid dehalogenase superfamily. CD73 belongs to a conserved superfamily of metallophosphodiesterases Homo sapiens
3.1.3.5 More the Isn1 polypeptide is a member of the HAD superfamily Saccharomyces cerevisiae
3.1.3.5 NMN 5'-nucleotidase
-
 Saccharomyces cerevisiae
3.1.3.5 Phm8
-
 Homo sapiens
3.1.3.5 Phm8
-
 Saccharomyces cerevisiae
3.1.3.5 Pho5
-
 Homo sapiens
3.1.3.5 Sdt1
-
 Homo sapiens
3.1.3.5 Sdt1
-
 Saccharomyces cerevisiae
3.1.3.5 UshA
-
 Homo sapiens
3.1.3.5 UshA
-
 Corynebacterium glutamicum

Expression

EC Number Organism Comment Expression
3.1.3.5 Corynebacterium glutamicum 5'-nucleotidase UshA is induced when phosphate is limiting up
3.1.3.5 Saccharomyces cerevisiae 5'-nucleotidases Sdt1 and Phm8 are induced at the transcript level by phosphate starvation up
3.1.3.5 Homo sapiens CD73 is highly induced by ischemia and hypoxia, and increased expression in cardiac ischemia is mediated by a positive feedback loop involving adenosine. Though CD73 is mainly regulated transcriptionally, cross linking of CD38, a cell surface glycoprotein with cyclic ADP-ribose hydrolase activity, causes upregulation of CD73 via export of protein to the cell surface that is independent of new transcription up

General Information

EC Number General Information Comment Organism
3.1.3.5 malfunction cytosolic 5'-nucleotidase cN-II is implicated in drug resistance in patients and dephosphorylates 5-fluoro-dUMP, 3'-azido-3-dTMP, 2'-chloro-2'-deoxyAMP, and related compounds, which represent the monophosphorylated forms of nucleoside prodrugs Homo sapiens
3.1.3.5 metabolism 5'-nucleotidases are involved in the purine and pyrimidine nucleotide metabolic pathways, overview. CD73 enzymatic activity is implicated in two major extracellular metabolic pathways with distinct physiological roles, extracellular adenosine nucleotide and NAD+ metabolism Homo sapiens
3.1.3.5 additional information CD73 is highly induced by ischemia and hypoxia, and increased expression in cardiac ischemia is mediated by a positive feedback loop involving adenosine Homo sapiens
3.1.3.5 physiological function 5'-nucleotidase activities regulate the quantity of nucleotides generated from both de novo and salvage pathways, overview. 5'-Nucleotidase designates a set of enzymes, which catalyze the hydrolysis of ribonucleoside and deoxyribonucleoside monophosphates into the corresponding nucleosides plus phosphate. 5'-Nucleotidases are classified according to subcellular localization, nucleobase specificity and their ability to hydrolyze deoxynucleoside monophosphate substrates. Membrane-bound 5'-nucleotidases are ectoenzymes principally involved in salvage of extracellular nucleosides, and often display a preference toward adenosine monophosphate, thereby modulating signal transduction cascades involving purinergic receptors. Cytosolic 5'-nucleotidases are members of the haloacid dehalogenase superfamily of enzymes, which are two-domain proteins containing a modified Rossman fold as the core and a variable cap structure. Extracellular and intracellular 5'-nucleotidase activities participate in purine and pyrimidine salvage to support balanced synthesis of nucleotides, which is critical for maintaining high fidelity DNA replication. The major physiological roles for CD73 in extracellular adenosine metabolism are severalfold, overview. cNII is subject to complex allosteric regulation Homo sapiens
3.1.3.5 physiological function 5'-nucleotidase UshA is required for growth when nucleotides are provided as the sole source of phosphate, a condition that is likely relevant to bacterial growth in the wild Corynebacterium glutamicum
3.1.3.5 physiological function ISN1 is responsible for virtually all IMP 5'-nucleotidase activity in budding yeast cells and is required for cells to release inosine into the culture medium Saccharomyces cerevisiae