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Literature summary extracted from

  • Huang, C.; Sadre-Bazzaz, K.; Shen, Y.; Deng, B.; Zhou, Z.; Tong, L.
    Crystal structure of the alpha6beta6 holoenzyme of propionyl-coenzyme A carboxylase (2010), Nature, 466, 1001-1005.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.4.1.3 expressed in Escherichia coli Ruegeria pomeroyi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.4.1.3 a PCC chimaera, containing the alpha-subunit of Ruegeria pomeroyi PCC and the beta-subunit of Roseobacter denitrificans PCC is crystallized by the microbatch method under paraffin oil, using 0.1 M HEPES (pH 8.0), 22% (w/v) PEG3350, 0.2 M NaCl and 16% (v/v) glycerol. Crystals of Ruegeria pomeroyi PCC are obtained at 20°C by the microbatch method under paraffin oil, using 0.2 M succinic acid (pH 6.5), 22% (w/v) benzamidine and 22% (w/v) PEG3000 Ruegeria pomeroyi
6.4.1.3 microbatch method under oil method Ruegeria pomeroyi

Protein Variants

EC Number Protein Variants Comment Organism
6.4.1.3 D440I the mutation does not change the substrate preference of the enzyme Ruegeria pomeroyi
6.4.1.3 G668R the mutation in the biotin carboxyl carrier protein domain abolishes biotinylation Ruegeria pomeroyi
6.4.1.3 R165Q the mutation disturbs the recognition of the adenine base of CoA Ruegeria pomeroyi
6.4.1.3 R165W the mutation disturbs the recognition of the adenine base of CoA Ruegeria pomeroyi
6.4.1.3 R399Q the mutation leads to a large loss in activity Ruegeria pomeroyi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.4.1.3 mitochondrion
-
 Ruegeria pomeroyi 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.3 750000
-
-
 Ruegeria pomeroyi
6.4.1.3 750000
-
 gel filtration Ruegeria pomeroyi

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.3 Ruegeria pomeroyi
-
-
-
6.4.1.3 Ruegeria pomeroyi Q5LUF3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.3 nickel affinity column chromatography and gel filtration Ruegeria pomeroyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.3 acetyl-CoA + CO2 the enzyme has a preference for propionyl-CoA over acetyl-CoA as the substrate Ruegeria pomeroyi ?
-
 ?
6.4.1.3 ATP + acetyl-CoA + HCO3- the enzyme has a preference for propanoyl-CoA over acetyl-CoA as the substrate Ruegeria pomeroyi ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3- the enzyme has a preference for propanoyl-CoA over acetyl-CoA as the substrate Ruegeria pomeroyi ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 propionyl-CoA + CO2 the enzyme has a preference for propionyl-CoA over acetyl-CoA as the substrate Ruegeria pomeroyi (S)-methylmalonyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.3 heterododecamer alpha6beta6-heterododecamer, the alpha-subunit contains the biotin carboxylase and biotin carboxyl carrier protein domains, whereas the beta-subunit supplies the carboxyltransferase activity Ruegeria pomeroyi
6.4.1.3 heterododecamer the alpha-subunit contains the biotin carboxylase and biotin carboxyl carrier protein domains, whereas the beta-subunit supplies the carboxyltransferase activity Ruegeria pomeroyi

Synonyms

EC Number Synonyms Comment Organism
6.4.1.3 PCC
-
 Ruegeria pomeroyi
6.4.1.3 propionyl-coenzyme A carboxylase
-
 Ruegeria pomeroyi

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.3 ATP
-
 Ruegeria pomeroyi
6.4.1.3 biotin
-
 Ruegeria pomeroyi

General Information

EC Number General Information Comment Organism
6.4.1.3 metabolism PCC is essential for the catabolism of the amino acids L-Thr, L-Val, L-Ile and L-Met, cholesterol and fatty acids with an odd number of carbon atoms Ruegeria pomeroyi