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Literature summary extracted from

  • Sun, D.; Lee, G.; Lee, J.H.; Kim, H.Y.; Rhee, H.W.; Park, S.Y.; Kim, K.J.; Kim, Y.; Kim, B.Y.; Hong, J.I.; Park, C.; Choy, H.E.; Kim, J.H.; Jeon, Y.H.; Chung, J.
    A metazoan ortholog of SpoT hydrolyzes ppGpp and functions in starvation responses (2010), Nat. Struct. Mol. Biol., 17, 1188-1194.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.7.2 recombinant expression of full-length enzyme in Escherichia coli and in HEK-293T cells Homo sapiens
3.1.7.2 recombinant expression of full-length enzyme in Escherichia coli and in S2 cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
3.1.7.2 additional information Mesh1 deletion by imprecise excision of a P-element from the Drosophila Mesh1 G3858 allele, resulting in the Drosophila Mesh1 null allele, Mesh1 5A3. The Mesh1 null mutant is much more susceptible than wild-type flies to death from amino acid starvation, and this susceptibility is also completely rescued by Mesh1 expression Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.7.2 Mg2+ activates, but less effective than Mn2+ Drosophila melanogaster
3.1.7.2 Mn2+ activates, required Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.7.2 additional information Homo sapiens only ppGpp, and no other nucleotide, is effectively hydrolyzed by human MESH1 ?
-
 ?
3.1.7.2 ppGpp + H2O Drosophila melanogaster
-
 ppG + diphosphate
-
 ?
3.1.7.2 ppGpp + H2O Homo sapiens
-
 ppG + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.7.2 Drosophila melanogaster
-
-
-
3.1.7.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.7.2 recombinant full-length enzyme from Escherichia coli Drosophila melanogaster
3.1.7.2 recombinant full-length enzyme from Escherichia coli Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.7.2 additional information only ppGpp, and no other nucleotide, is effectively hydrolyzed by human MESH1 Homo sapiens ?
-
 ?
3.1.7.2 additional information activity detection by usage of the chemosensor pyrene and bis(Zn2+-dipicolylamine), which generates fluorescence at 470 nm when it specifically binds to ppGpp, overview Drosophila melanogaster ?
-
 ?
3.1.7.2 additional information activity detection by usage of the chemosensor pyrene and bis(Zn2+-dipicolylamine), which generates fluorescence at 470 nm when it specifically binds to ppGpp, overview Homo sapiens ?
-
 ?
3.1.7.2 ppGpp + H2O
-
 Drosophila melanogaster ppG + diphosphate
-
 ?
3.1.7.2 ppGpp + H2O
-
 Homo sapiens ppG + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.7.2 More MESH1 consists only of the hydrolase domain of bacterial SpoT and lack the synthetase and regulatory domains, structure comparisons, overview Drosophila melanogaster
3.1.7.2 More MESH1 consists only of the hydrolase domain of bacterial SpoT and lack the synthetase and regulatory domains, structure comparisons, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.7.2 HDDC3
-
 Homo sapiens
3.1.7.2 MESH1
-
 Drosophila melanogaster
3.1.7.2 MESH1
-
 Homo sapiens
3.1.7.2 metazoan SpoT homolog-1 Mesh1 consists only of the hydrolase domain of bacterial SpoT and lack the synthetase and regulatory domains Drosophila melanogaster
3.1.7.2 metazoan SpoT homolog-1 Mesh1 consists only of the hydrolase domain of bacterial SpoT and lack the synthetase and regulatory domains Homo sapiens
3.1.7.2 ppGpp hydrolase
-
 Drosophila melanogaster
3.1.7.2 ppGpp hydrolase
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
3.1.7.2 malfunction Mesh1 deletion impairs starvation resistance in Drosophila, overview Drosophila melanogaster
3.1.7.2 additional information crucial residues for the ppGpp hydrolysis activity of Mesh1 are Arg24, Glu65, Asp66 and Asn126 Drosophila melanogaster

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.7.2 9.46
-
 ppGpp pH not specified in the publication, temperature not specified in the publication Homo sapiens
3.1.7.2 9.58
-
 ppGpp pH not specified in the publication, temperature not specified in the publication Drosophila melanogaster