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Literature summary extracted from

  • Young, S.A.; Smith, T.K.
    The essential neutral sphingomyelinase is involved in the trafficking of the variant surface glycoprotein in the bloodstream form of Trypanosoma brucei (2010), Mol. Microbiol., 76, 1461-1482.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.4.12 drug development TbnSMase is therefore genetically validated as a drug target against African trypanosomes, and suggests that interfering with the endocytic transport of variant surface glycoprotein is a highly desirable strategy for drug development against African trypanosomasis Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 nSMase overexpression as GST-tagged enzyme in Escherichia coli strain C43 (DE3) Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 membrane
-
 Trypanosoma brucei 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Mg2+ required Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.12 81000
-
 x * 81000, recombinant GST-tagged nSMase, SDS-PAGE Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 a sphingomyelin + H2O Trypanosoma brucei
-
 a ceramide + choline phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Trypanosoma brucei
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 a sphingomyelin + H2O
-
 Trypanosoma brucei a ceramide + choline phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.12 ? x * 81000, recombinant GST-tagged nSMase, SDS-PAGE Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 neutral sphingomyelinase
-
 Trypanosoma brucei

General Information

EC Number General Information Comment Organism
3.1.4.12 malfunction loss of nSMase activity leads to a Big-Eye-like phenotype, and affect s protein trafficking, as well as VSG trafficking, overview Trypanosoma brucei
3.1.4.12 metabolism the essential neutral sphingomyelinase is involved in the trafficking of the variant surface glycoprotein in the bloodstream form of Trypanosoma brucei Trypanosoma brucei
3.1.4.12 physiological function the neutral sphingomyelinase is essential for growth and survival of the parasite. The formation of ceramide in the endoplasmic reticulum affects post-Golgi sorting and rate of deposition of newly synthesized GPI-anchored variant surface glycoprotein on the cell surface Trypanosoma brucei