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Literature summary extracted from
- 3-Ketosteroid 9alpha-hydroxylase is an essential factor in the pathogenesis of Mycobacterium tuberculosis (2010), Mol. Microbiol., 75, 107-121.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.99.24 | genes kshA or Rv3526, and kshB or Rv3571 | Mycobacterium tuberculosis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.99.24 | additional information | gene disruption by a two-step mutagenesis strategy, the constructed DELTAkshA and DELTAkshB deletion mutants of strain H37Rv are unable to use cholesterol and 4-androstene-3,17-dione as primary carbon and energy sources, and are also unable to metabolize the steroid substrate 5alpha-androstane-3,17-dione in contrast to the wild-type enzyme, phenotypes, overview | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.24 | 1,4-androstadiene-3,17-dione + 2 O2 + 2 H+ | Mycobacterium tuberculosis | - |
9-hydroxy-1,4-androstadiene-3,17-dione + H2O2 | - |
? |  |
| 1.14.99.24 | 1,4-androstadiene-3,17-dione + 2 O2 + 2 H+ | Mycobacterium tuberculosis H37Rv | - |
9-hydroxy-1,4-androstadiene-3,17-dione + H2O2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.30 | Mycobacterium tuberculosis | - |
- |
- |
| 1.14.15.30 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
| 1.14.99.24 | Mycobacterium tuberculosis | - |
genes kshA or Rv3526, and kshB or Rv3571 | - |
| 1.14.99.24 | Mycobacterium tuberculosis H37Rv | - |
genes kshA or Rv3526, and kshB or Rv3571 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.30 | 5alpha-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis | 9alpha-hydroxy-5alpha-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
| 1.14.15.30 | 5alpha-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis H37Rv | 9alpha-hydroxy-5alpha-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
| 1.14.15.30 | androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis | 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
| 1.14.15.30 | androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycobacterium tuberculosis H37Rv | 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
| 1.14.99.24 | 1,4-androstadiene-3,17-dione + 2 O2 + 2 H+ | - |
Mycobacterium tuberculosis | 9-hydroxy-1,4-androstadiene-3,17-dione + H2O2 | - |
? | |
| 1.14.99.24 | 1,4-androstadiene-3,17-dione + 2 O2 + 2 H+ | - |
Mycobacterium tuberculosis H37Rv | 9-hydroxy-1,4-androstadiene-3,17-dione + H2O2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.99.24 | More | KSH is a two-component Rieske-type oxygenase comprised of an oxygenase component KshA and a reductase component KshB | Mycobacterium tuberculosis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.15.30 | 3-ketosteroid 9alpha-hydroxylase | - |
Mycobacterium tuberculosis |
| 1.14.15.30 | KSH | 3-ketosteroid 9alpha-hydroxylase is comprised of KshA (oxygenase) and KshB (oxygenase-reductase) | Mycobacterium tuberculosis |
| 1.14.99.24 | 3-ketosteroid 9alpha-hydroxylase | - |
Mycobacterium tuberculosis |
| 1.14.99.24 | KSH | - |
Mycobacterium tuberculosis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.15.30 | malfunction | DELTAkshA and DELTAkshB deletion mutants are unable to use cholesterol and 4-androstene-3,17-dione as primary carbon and energy sources and are unable to metabolize 5alpha-androstane-3,17-dione. The deletion of either of these genes leads to rapid death of the microorganism in murine infection models and in macrophages | Mycobacterium tuberculosis |
| 1.14.15.30 | physiological function | 3-ketosteroid 9alpha-hydroxylase is an essential factor in the pathogenesis of Mycobacterium tuberculosis | Mycobacterium tuberculosis |
| 1.14.99.24 | metabolism | 3-ketosteroid 9alpha-hydroxylase is a critical pathogenicity factor of strainH37Rv involved in steroid and cholesterol catabolism | Mycobacterium tuberculosis |
| 1.14.99.24 | additional information | DELTAkshA and DELTAkshB deletion mutants of strain H37Rv are unable to use cholesterol and 4-androstene-3,17-dione as primary carbon and energy sources, and are also unable to metabolize the steroid substrate 5alpha-androstane-3,17-dione in contrast to the wild-type enzyme. The deletion of either of these genes leads to rapid death of the microorganism in murine infection models and in macrophages, showing that kshA and kshB are essential factors for Mycobacterium tuberculosis pathogenesis | Mycobacterium tuberculosis |
| 1.14.99.24 | physiological function | genes kshA and kshB are essential factors for Mycobacterium tuberculosis pathogenesis | Mycobacterium tuberculosis |
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