EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.26 | expression of wild-type and mutant PylRS in Escherichia coli | Methanosarcina mazei |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.1.1.26 | additional information | evolving a PylRS mutant that specifically acylates tRNAPylCUA with L-phenylalanine, p-iodo-L-phenylalanine and p-bromo-L-phenylalanine by standard positive and negative selections of the pRS1 plasmid library in Escherichia coli. The pRS1 plasmid library contains the Methanosarcina mazei PylRS gene with randomization at six active-site residues, L305, Y306, L309, N346, C348, and W417, method, overview | Methanosarcina mazei |
6.1.1.26 | additional information | usage of evolved pyrrolysyl-tRNA synthetase-tRNAPylCUA pairs for genetic incorporation of L-phenylalanine, p-iodo-L-phenylalanine and p-bromo-L-phenylalanine into proteins at amber mutation sites in Escherichia coli | Methanocaldococcus jannaschii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.26 | Methanocaldococcus jannaschii | - |
- |
- |
6.1.1.26 | Methanosarcina mazei | - |
- |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.26 | pyrrolysyl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
6.1.1.26 | pyrrolysyl-tRNA synthetase | - |
Methanosarcina mazei |