Any feedback?
Literature summary extracted from
- Oxidative-stress-inducible qorA encodes an NADPH-dependent quinone oxidoreductase catalysing a one-electron reduction in Staphylococcus aureus (2003), Microbiology, 149, 389-398.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.6.5.5 | expression in Escherichia coli | Staphylococcus aureus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.5.5 | 0.0491 | - |
9,10-phenanthrenequinone | pH 7.5, 25°C | Staphylococcus aureus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.6.5.5 | 36300 | - |
x * 36300, calculated and co-purification results | Staphylococcus aureus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.5.5 | Staphylococcus aureus | A0A0H3JVE7 | - |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.6.5.5 | 14.8 | - |
substrate menadione, pH 7.5, 25°C | Staphylococcus aureus |
| 1.6.5.5 | 311 | - |
substrate 9,10-phenanthrenequinone, pH 7.5, 25°C | Staphylococcus aureus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.5.5 | 9,10-phenanthrenequinone + NADPH + H+ | one-electron reduction mechanism. Concomitantly with NADPH consumption, generation of superoxide is observed | Staphylococcus aureus | 9,10-phenanthrenequinol + NADP+ | - |
? | |
| 1.6.5.5 | menadione + NADPH + H+ | - |
Staphylococcus aureus | menadiol + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.6.5.5 | multimer | x * 36300, calculated and co-purification results | Staphylococcus aureus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.6.5.5 | QorA | - |
Staphylococcus aureus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.5.5 | additional information | no cofactor: NADH. Sequence does not contain a flavin-binding motif | Staphylococcus aureus | |
| 1.6.5.5 | NADPH | - |
Staphylococcus aureus | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.6.5.5 | Staphylococcus aureus | calculated | - |
5.2 |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.6.5.5 | Staphylococcus aureus | induced in presence of 9,10-phenanthrenequinone and by oxidative stress | up |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
