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Literature summary extracted from

  • Drzewiecki, K.; Angelov, A.; Ballschmiter, M.; Tiefenbach, K.J.; Sterner, R.; Liebl, W.
    Hyperthermostable acetyl xylan esterase (2010), Microb. Biotechnol., 3, 84-92.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.72 gene axeA, the acetylxylan esterase is encoded in a gene cluster for xylan degradation, sequence comparisons Thermotoga maritima

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Thermotoga maritima
-
 gene axeA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Thermotoga maritima 4-nitrophenol + acetate
-
 ?
3.1.1.72 cephalosporin C + H2O deacetylation Thermotoga maritima deacetylcephalosporin C + acetate
-
 ?
3.1.1.72 D-glucose penta-acetate + H2O deacetylation Thermotoga maritima ?
-
 ?
3.1.1.72 xylan + H2O deacetylation of acetylated oat spelt xylan and DMSO-extracted beechwood xylan Thermotoga maritima ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.72 homohexamer gel filtration and crystal structure, a dimeric form is also present Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.1.1.72 acetyl xylan esterase
-
 Thermotoga maritima
3.1.1.72 AxeA
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.72 90
-
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.72 additional information
-
 AxeA shows multiphasic unfolding behaviour with two exothermic peaks at 100-104°C and 107.5°C Thermotoga maritima
3.1.1.72 90
-
 half-life is 67 h in absence of substrate Thermotoga maritima
3.1.1.72 98
-
 half-life is 13 h in absence of substrate Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.72 6.5
-
-
 Thermotoga maritima