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Literature summary extracted from

  • Bhatt, R.; Patel, K.; Trivedi, U.
    Purification and properties of extracellular poly(3-hydroxybutyrate) depolymerase produced by Aspergillus fumigatus 202 (2010), J. Polym. Environ., 18, 141-147.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.75 dithiothreitol inactivation of the purified enzyme Aspergillus fumigatus
3.1.1.75 Fe2+ 88% inhibition at 5 mM Aspergillus fumigatus
3.1.1.75 Hg2+ complete inhibition at 5 mM Aspergillus fumigatus
3.1.1.75 N-bromosuccinimide inactivation of the purified enzyme Aspergillus fumigatus
3.1.1.75 Triton X-100
-
 Aspergillus fumigatus
3.1.1.75 Tween 20
-
 Aspergillus fumigatus
3.1.1.75 Tween 80
-
 Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 extracellular
-
 Aspergillus fumigatus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.75 Ca2+ 1.5fold activation at 5 mM Aspergillus fumigatus
3.1.1.75 Mg2+ 1.5fold activation at 5 mM Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.75 63744
-
 x * 63744, SDS-PAGE Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.75 poly(3-hydroxybutyrate) + H2O Aspergillus fumigatus
-
 ?
-
 ?
3.1.1.75 poly(3-hydroxybutyrate) + H2O Aspergillus fumigatus 202
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Aspergillus fumigatus
-
 a thermotolerant fungal soil isolate
-
3.1.1.75 Aspergillus fumigatus 202
-
 a thermotolerant fungal soil isolate
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.75 no glycoprotein
-
 Aspergillus fumigatus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75 native extracellular enzyme 20.62fold by acetone precipitation and gel filtration Aspergillus fumigatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.75 additional information maximum PHB depolymerase production is obtained at the end of 48 h with initial medium pH 7.0 and 45°C in Bushnell Haas Minerals medium containing PHB as sole source of carbon Aspergillus fumigatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.75 additional information
-
 4.5512 mg PHB/min, purified enzyme Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 poly(3-hydroxybutyrate) + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.1.1.75 poly(3-hydroxybutyrate) + H2O
-
 Aspergillus fumigatus 202 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.75 ? x * 63744, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.75 PHB depolymerase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.75 45
-
-
 Aspergillus fumigatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.75 37 70 activity range Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.75 55
-
 the purified enzyme retains 56.01% of maximal activity up to 55°C Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.75 9
-
-
 Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.75 3 10 activity range Aspergillus fumigatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.75 Aspergillus fumigatus isoelectric focusing
-
 4.2