EC Number | Cloned (Comment) | Organism |
---|---|---|
5.1.1.3 | expressed in Escherichia coli BL21(DE3) cells | Bacillus anthracis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.1.1.3 | K106A | the isozyme RacE2 mutant is insensitive to dipicolinic acid | Bacillus anthracis |
5.1.1.3 | S207A | the isozyme RacE2 mutant is insensitive to dipicolinic acid | Bacillus anthracis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.1.1.3 | dipicolinic acid | allosteric inhibitor of both Bacillus anthracis glutamate racemase isozymes which exhibits low micromolar inhibition with clear noncompetitive behavior | Bacillus anthracis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.1.3 | Bacillus anthracis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.1.3 | ammonium sulfate precipitation, Co2+-affinity column chromatography, and anion-exchange chromatography | Bacillus anthracis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.1.3 | D-glutamate | - |
Bacillus anthracis | L-glutamate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.1.1.3 | RacE1 | isozyme | Bacillus anthracis |
5.1.1.3 | RacE2 | isozyme | Bacillus anthracis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.1.1.3 | 8 | - |
- |
Bacillus anthracis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.1.3 | 0.075 | - |
dipicolinic acid | isozyme RacE1, pH and temperature not specified in the publication | Bacillus anthracis | |
5.1.1.3 | 0.092 | - |
dipicolinic acid | isozyme RacE2, pH and temperature not specified in the publication | Bacillus anthracis | |
5.1.1.3 | 1.236 | - |
dipicolinic acid | RacE2 mutant enzyme S207A, pH and temperature not specified in the publication | Bacillus anthracis | |
5.1.1.3 | 2.342 | - |
dipicolinic acid | RacE2 mutant enzyme K106A, pH and temperature not specified in the publication | Bacillus anthracis |