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Literature summary extracted from

  • Somboon, T.; Gleeson, M.P.; Hannongbua, S.
    Insight into the reaction mechanism of cis,cis-muconate lactonizing enzymes: a DFT QM/MM study (2012), J. Mol. Model., 18, 525-531.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.5.1.1 Mg2+ Mg2+ binds deep within a non-solvent exposed cavity, coordinated by 2 aspartate and 1 glutamic acid residues, a single water molecule and the substrate, in a distorted octahedral form. The carboxylate of cis,cis-muconate binds across the Mg2+ ion in conformations that display mirror-like symmetry in the two different proteins Mycolicibacterium smegmatis
5.5.1.1 Mg2+ Mg2+ binds deep within a non-solvent exposed cavity, coordinated by 2 aspartate and 1 glutamic acid residues, a single water molecule and the substrate, in a distorted octahedral form. The carboxylate of cis,cis-muconate binds across the Mg2+ ion in conformations that display mirror-like symmetry in the two different proteins Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.1 Mycolicibacterium smegmatis
-
-
-
5.5.1.1 Pseudomonas fluorescens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.5.1.1 (+)-muconolactone = cis,cis-muconate reaction mechanism, DFT QM/MM calculations and structure-function relationship, overview Mycolicibacterium smegmatis
a
5.5.1.1 (+)-muconolactone = cis,cis-muconate reaction mechanism, DFT QM/MM calculations and structure-function relationship, overview Pseudomonas fluorescens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.1 cis,cis-hexadienedioate conversion of cis,cis-muconate to muconolactone, two proximal Lys residues exist in the active sites, which can potentially lead, via a single intermediate form, to the formation of either the syn- or anti-products. The enzyme from Mycobacterium smegmatis forms a syn-product, overview Mycolicibacterium smegmatis 2,5-dihydro-5-oxofuran-2-acetate
-
 r
5.5.1.1 cis,cis-hexadienedioate conversion of cis,cis-muconate to muconolactone, two proximal Lys residues exist in the active sites, which can potentially lead, via a single intermediate form, to the formation of either the syn- or anti-products. The enzyme from Pseudomonas fluorescens forms an anti-product, overview Pseudomonas fluorescens 2,5-dihydro-5-oxofuran-2-acetate
-
 r

Subunits

EC Number Subunits Comment Organism
5.5.1.1 More TIM barrel-like alpha/beta protein fold Mycolicibacterium smegmatis
5.5.1.1 More TIM barrel-like alpha/beta protein fold Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
5.5.1.1 anti-MLE
-
 Mycolicibacterium smegmatis
5.5.1.1 cis,cis-muconate lactonizing enzyme
-
 Mycolicibacterium smegmatis
5.5.1.1 cis,cis-muconate lactonizing enzyme
-
 Pseudomonas fluorescens
5.5.1.1 MLE
-
 Mycolicibacterium smegmatis
5.5.1.1 MLE
-
 Pseudomonas fluorescens
5.5.1.1 syn-MLE
-
 Pseudomonas fluorescens