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Literature summary extracted from

  • Bains, J.; Kaufman, L.; Farnell, B.; Boulanger, M.J.
    A product analog bound form of 3-oxoadipate-enol-lactonase (PcaD) reveals a multifunctional role for the divergent cap domain (2011), J. Mol. Biol., 406, 649-658.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.24 expressed in Escherichia coli Paraburkholderia xenovorans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.24 in complex with levulinic acid Paraburkholderia xenovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.24 57600
-
calculated from amino acid sequence Paraburkholderia xenovorans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.24 Paraburkholderia xenovorans Q13KT2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.24 Ni2+ affinity column chromatography and Superdex75 gel filtration Paraburkholderia xenovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.24 3-oxoadipate-enol-lactone + H2O also known as beta-ketoadipate-enol-lactone Paraburkholderia xenovorans 3-oxoadipate
-
?
3.1.1.24 4-hydroxy-3-pentenoic acid gamma-lactone + H2O
-
Paraburkholderia xenovorans 4-oxopentanoic acid i.e. levulinic acid ?

Subunits

EC Number Subunits Comment Organism
3.1.1.24 homodimer x-ray crystallography Paraburkholderia xenovorans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.24 3-oxoadipate-enol-lactonase
-
Paraburkholderia xenovorans
3.1.1.24 ELH
-
Paraburkholderia xenovorans
3.1.1.24 enol-lactone hydrolase
-
Paraburkholderia xenovorans
3.1.1.24 PcaD
-
Paraburkholderia xenovorans