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Literature summary extracted from

  • Xu, K.; Tang, X.; Gai, Y.; Mehmood, M.; Xiao, X.; Wang, F.
    Molecular characterization of cold-inducible beta-galactosidase from Arthrobacter sp. ON14 isolated from Antarctica (2011), J. Microbiol. Biotechnol., 21, 236-242.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Cu2+ inactivates enzymatic activity Arthrobacter sp.
3.2.1.23 Zn2+ reduces enzymatic activity Arthrobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 K+ activates enzymatic activity Arthrobacter sp.
3.2.1.23 Mn2+ activates enzymatic activity Arthrobacter sp.
3.2.1.23 Na+ activates enzymatic activity Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 116000
-
SDS-PAGE Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Arthrobacter sp. D9IU57 ON14
-
3.2.1.23 Arthrobacter sp. D9IU58 ON14
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
Arthrobacter sp. 2-nitrophenol + beta-D-galactose
-
?
3.2.1.23 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside + H2O
-
Arthrobacter sp. 5-bromo-4-chloro-indol + beta-D-galactose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 beta-galactosidase
-
Arthrobacter sp.
3.2.1.23 GALA
-
Arthrobacter sp.
3.2.1.23 GALB
-
Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 15
-
-
Arthrobacter sp.
3.2.1.23 37
-
-
Arthrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 45 50 an enzyme stability assay reveals that the activity of GalA is significantly decreased when it is incubated at 45°C for 2 h, and all its activity is lost when it is incubated at 50°C Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 8
-
-
Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 6 9 75% activity Arthrobacter sp.

Expression

EC Number Organism Comment Expression
3.2.1.23 Arthrobacter sp. expression is inhibited at low temperature down
3.2.1.23 Arthrobacter sp. expression is induced at high temperature up
3.2.1.23 Arthrobacter sp. expression of galA is highly induced at a low temperature and repressed at a high temperature when lactose is used as the sole carbon source up