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Literature summary extracted from

  • Ibarguren, M.; Lopez, D.J.; Montes, L.R.; Sot, J.; Vasil, A.I.; Vasil, M.L.; Goni, F.M.; Alonso, A.
    Imaging the early stages of phospholipase C/sphingomyelinase activity on vesicles containing coexisting ordered-disordered and gel-fluid domains (2011), J. Lipid Res., 52, 635-645.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.3 T178A site-directed mutagenesis, hydrolytically inactive mutant. The PlcHR 2 T178A mutant is also unable to induce vesicle aggregation or release intravesicular aqueous contents in contrast to the wild-type enzyme Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 additional information activity measurement with substrate on giant unilamellar lipid vesicles. Enzyme binding to the vesicles appears to be a cooperative process and is irreversible, binding analysis using wild-type enzyme and catalytically inactive enzyme mutant T178A. After the initial cluster of bound enzyme is detected, further binding and catalytic activity follow rapidly. The enzyme preferentially binds the more disordered domains, and, in most cases, the catalytic activity causes the disordering of the other domains, at a further stage of lipid hydrolysis, lipid aggregates are formed and vesicles disintegrate, overview Pseudomonas aeruginosa ?
-
?
3.1.4.3 phosphatidylcholine + H2O
-
Pseudomonas aeruginosa 1,2-diacyl-sn-glycerol + choline phosphate
-
?
3.1.4.3 phosphatidylethanolamine + H2O
-
Pseudomonas aeruginosa 1,2-diacyl-sn-glycerol + ethanolamine phosphate
-
?
3.1.4.3 sphingomyelin + H2O
-
Pseudomonas aeruginosa N-acylsphingosine + choline phosphate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.3 28
-
assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.3 7.4
-
assay at Pseudomonas aeruginosa