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Literature summary extracted from

  • Munoz, A.; Bannenberg, G.L.; Montero, O.; Cabello-Diaz, J.M.; Piedras, P.; Pineda, M.
    An alternative pathway for ureide usage in legumes: enzymatic formation of a ureidoglycolate adduct in Cicer arietinum and Phaseolus vulgaris (2011), J. Exp. Bot., 62, 307-318.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.B3 4
-
phenylhydrazine pH 7.8, 30°C Cicer arietinum
2.3.2.B3 4
-
phenylhydrazine at pH 7.8 and 22°C Cicer arietinum
2.3.2.B3 8.5
-
phenylhydrazine pH 7.8, 30°C Phaseolus vulgaris
2.3.2.B3 8.5
-
phenylhydrazine at pH 7.8 and 22°C Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.B3 (S)-ureidoglycolate + phenylhydrazine Phaseolus vulgaris
-
ureidoglycoylphenylhydrazide the product decomposes non-enzymatically to glyoxylate and urea ?
2.3.2.B3 (S)-ureidoglycolate + phenylhydrazine Cicer arietinum
-
ureidoglycoylphenylhydrazide the product decomposes non-enzymatically to glyoxylate and urea ?
2.3.2.B3 phenylhydrazine + (S)-ureidoglycolate Phaseolus vulgaris
-
1-[(1S)-hydroxy-2-oxo-2-(2-phenylhydrazinyl)ethyl]urea + H2O
-
?
2.3.2.B3 phenylhydrazine + (S)-ureidoglycolate Cicer arietinum
-
1-[(1S)-hydroxy-2-oxo-2-(2-phenylhydrazinyl)ethyl]urea + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.B3 Cicer arietinum
-
-
-
2.3.2.B3 Cicer arietinum
-
chickpea
-
2.3.2.B3 Phaseolus vulgaris
-
-
-
2.3.2.B3 Phaseolus vulgaris
-
french bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.B3
-
Phaseolus vulgaris
2.3.2.B3
-
Cicer arietinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.B3 (S)-ureidoglycolate + phenylhydrazine
-
Phaseolus vulgaris ureidoglycoylphenylhydrazide the product decomposes non-enzymatically to glyoxylate and urea ?
2.3.2.B3 (S)-ureidoglycolate + phenylhydrazine
-
Cicer arietinum ureidoglycoylphenylhydrazide the product decomposes non-enzymatically to glyoxylate and urea ?
2.3.2.B3 additional information enzyme catalyses the transfer of the ureidoglycolyl group to phenylhydrazine to produce ureidoglycolyl phenylhydrazide, which may degrade non-enzymatically to glyoxylate phenylhydrazone and urea Phaseolus vulgaris ?
-
?
2.3.2.B3 additional information enzyme catalyses the transfer of the ureidoglycolyl group to phenylhydrazine to produce ureidoglycolyl phenylhydrazide, which may degrade non-enzymatically to glyoxylate phenylhydrazone and urea Cicer arietinum ?
-
?
2.3.2.B3 phenylhydrazine + (S)-ureidoglycolate
-
Phaseolus vulgaris 1-[(1S)-hydroxy-2-oxo-2-(2-phenylhydrazinyl)ethyl]urea + H2O
-
?
2.3.2.B3 phenylhydrazine + (S)-ureidoglycolate
-
Cicer arietinum 1-[(1S)-hydroxy-2-oxo-2-(2-phenylhydrazinyl)ethyl]urea + H2O
-
?
2.3.2.B3 ureidoglycolate + phenylhydrazine reaction requires phenylhydrazine, no production of glyoxylate Phaseolus vulgaris ureidoglycolyl phenylhydrazide + H2O product is detected as its two-electron oxidized hydrazine derivative ?
2.3.2.B3 ureidoglycolate + phenylhydrazine reaction requires phenylhydrazine, no production of glyoxylate Cicer arietinum ureidoglycolyl phenylhydrazide + H2O product is detected as its two-electron oxidized hydrazine derivative ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.B3 ureidoglycolate urea-lyase
-
Phaseolus vulgaris
2.3.2.B3 ureidoglycolate urea-lyase
-
Cicer arietinum