EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.83 | membrane | - |
Catharanthus roseus | 16020 | - |
1.14.14.83 | membrane | - |
Tabernaemontana divaricata | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.83 | 56000 | - |
x * 56000, SDS-PAGE | Catharanthus roseus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.83 | geraniol + [reduced NADPH-hemoprotein reductase] + O2 | Catharanthus roseus | - |
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.83 | geraniol + [reduced NADPH-hemoprotein reductase] + O2 | Tabernaemontana divaricata | - |
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.83 | nerol + [reduced NADPH-hemoprotein reductase] + O2 | Catharanthus roseus | cis-isomer of geraniol | 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.83 | nerol + [reduced NADPH-hemoprotein reductase] + O2 | Tabernaemontana divaricata | cis-isomer of geraniol | 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.83 | Catharanthus roseus | - |
- |
- |
1.14.14.83 | no activity in Tabernaemontana pandacaqui | - |
- |
- |
1.14.14.83 | Tabernaemontana divaricata | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.83 | native cholate-solubilized G10H about 8.3fold from suspension cell membranes by anion exchange and hydroxyapatite chromatography, and 2-aminooctyl agarose and a second step of hydrophobic interaction chromatography to homogeneity. Separation from the NADPH-cytochrome P-450 reductase, EC 1.6.2.4 | Catharanthus roseus |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.14.14.83 | reconstitution of the purified enzyme by addition of NADPH-cytochrome P-450 reductase, EC 1.6.2.4, and lipid extract | Catharanthus roseus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.83 | cell suspension culture | - |
Catharanthus roseus | - |
1.14.14.83 | cell suspension culture | during the first week of growth, no activity in the stationary growth phase | Tabernaemontana divaricata | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.14.83 | 8.66 | - |
purified enzyme, pH 7.6, temperature not specified in the publication | Catharanthus roseus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.83 | geraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Catharanthus roseus | 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.83 | geraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Tabernaemontana divaricata | 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.83 | nerol + [reduced NADPH-hemoprotein reductase] + O2 | cis-isomer of geraniol | Catharanthus roseus | 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.83 | nerol + [reduced NADPH-hemoprotein reductase] + O2 | cis-isomer of geraniol | Tabernaemontana divaricata | 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.83 | ? | x * 56000, SDS-PAGE | Catharanthus roseus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.83 | G10H | - |
Catharanthus roseus |
1.14.14.83 | G10H | - |
Tabernaemontana divaricata |
1.14.14.83 | monoterpene hydroxylase | - |
Catharanthus roseus |
1.14.14.83 | monoterpene hydroxylase | - |
Tabernaemontana divaricata |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.83 | 7.6 | - |
assay at | Catharanthus roseus |
1.14.14.83 | 7.6 | - |
assay at | Tabernaemontana divaricata |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.83 | cytochrome P450 | - |
Tabernaemontana divaricata | |
1.14.14.83 | cytochrome P450 | 4.72 nmol/mg | Catharanthus roseus | |
1.14.14.83 | FAD | - |
Catharanthus roseus | |
1.14.14.83 | FAD | - |
Tabernaemontana divaricata |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.83 | additional information | geraniol 10-hydroxylase is a cytochrome P450 monooxygenase | Catharanthus roseus |
1.14.14.83 | additional information | geraniol 10-hydroxylase is a cytochrome P450 monooxygenase | Tabernaemontana divaricata |