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Literature summary extracted from

  • Meijer, A.H.; De Waal, A.; Verpoorte, R.
    Purification of the cytochrome P-450 enzyme geraniol 10-hydroxylase from cell cultures of Catharanthus roseus (1993), J. Chromatogr. A, 635, 237-249.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.83 membrane
-
 Catharanthus roseus 16020
-
1.14.14.83 membrane
-
 Tabernaemontana divaricata 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.83 56000
-
 x * 56000, SDS-PAGE Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus
-
 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2 Tabernaemontana divaricata
-
 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.83 nerol + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus cis-isomer of geraniol 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.83 nerol + [reduced NADPH-hemoprotein reductase] + O2 Tabernaemontana divaricata cis-isomer of geraniol 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.83 Catharanthus roseus
-
-
-
1.14.14.83 no activity in Tabernaemontana pandacaqui
-
-
-
1.14.14.83 Tabernaemontana divaricata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.83 native cholate-solubilized G10H about 8.3fold from suspension cell membranes by anion exchange and hydroxyapatite chromatography, and 2-aminooctyl agarose and a second step of hydrophobic interaction chromatography to homogeneity. Separation from the NADPH-cytochrome P-450 reductase, EC 1.6.2.4 Catharanthus roseus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.14.14.83 reconstitution of the purified enzyme by addition of NADPH-cytochrome P-450 reductase, EC 1.6.2.4, and lipid extract Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.83 cell suspension culture
-
 Catharanthus roseus
-
1.14.14.83 cell suspension culture during the first week of growth, no activity in the stationary growth phase Tabernaemontana divaricata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.83 8.66
-
 purified enzyme, pH 7.6, temperature not specified in the publication Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Catharanthus roseus 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Tabernaemontana divaricata 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.83 nerol + [reduced NADPH-hemoprotein reductase] + O2 cis-isomer of geraniol Catharanthus roseus 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.83 nerol + [reduced NADPH-hemoprotein reductase] + O2 cis-isomer of geraniol Tabernaemontana divaricata 10-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.83 ? x * 56000, SDS-PAGE Catharanthus roseus

Synonyms

EC Number Synonyms Comment Organism
1.14.14.83 G10H
-
 Catharanthus roseus
1.14.14.83 G10H
-
 Tabernaemontana divaricata
1.14.14.83 monoterpene hydroxylase
-
 Catharanthus roseus
1.14.14.83 monoterpene hydroxylase
-
 Tabernaemontana divaricata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.83 7.6
-
 assay at Catharanthus roseus
1.14.14.83 7.6
-
 assay at Tabernaemontana divaricata

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.83 cytochrome P450
-
 Tabernaemontana divaricata
1.14.14.83 cytochrome P450 4.72 nmol/mg Catharanthus roseus
1.14.14.83 FAD
-
 Catharanthus roseus
1.14.14.83 FAD
-
 Tabernaemontana divaricata

General Information

EC Number General Information Comment Organism
1.14.14.83 additional information geraniol 10-hydroxylase is a cytochrome P450 monooxygenase Catharanthus roseus
1.14.14.83 additional information geraniol 10-hydroxylase is a cytochrome P450 monooxygenase Tabernaemontana divaricata