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Literature summary extracted from

  • Juhl, P.B.; Doderer, K.; Hollmann, F.; Thum, O.; Pleiss, J.
    Engineering of Candida antarctica lipase B for hydrolysis of bulky carboxylic acid esters (2010), J. Biotechnol., 150, 474-480.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3
-
 Moesziomyces antarcticus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.3 D134S the mutant shows increased activity compared to the wild type enzyme Moesziomyces antarcticus
3.1.1.3 I189L the mutant shows wild type activity Moesziomyces antarcticus
3.1.1.3 Q157A the mutant shows increased activity compared to the wild type enzyme Moesziomyces antarcticus
3.1.1.3 Q157I the mutant shows decreased activity compared to the wild type enzyme Moesziomyces antarcticus
3.1.1.3 Q157L the mutant shows decreased activity compared to the wild type enzyme Moesziomyces antarcticus
3.1.1.3 Q157N the mutant shows decreased activity compared to the wild type enzyme Moesziomyces antarcticus
3.1.1.3 Q157S the mutant shows increased activity compared to the wild type enzyme Moesziomyces antarcticus
3.1.1.3 Q157T the mutant shows increased activity compared to the wild type enzyme Moesziomyces antarcticus
3.1.1.3 T138A the mutant shows decreased activity compared to the wild type enzyme Moesziomyces antarcticus
3.1.1.3 T138S the mutant shows a 5fold increase in hydrolysis of isononanoic acid ethyl ester, but not toward sterically more demanding 2-ethyl hexanoic acid ethyl ester Moesziomyces antarcticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 50000
-
 x * 50000, about 50000 Da, SDS-PAGE Moesziomyces antarcticus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Moesziomyces antarcticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3
-
 Moesziomyces antarcticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 additional information
-
 the wild type enzyme shows 741, 3.1 and 0.5 units/mg specific activity towards methyl caprylate, isononanoic acid ethyl ester and 2-ethyl hexanonic acid ethyl ester, respectively. Mutant enzyme T138S shows 427, 15.3 and 0.6 units/mg specific activity towards methyl caprylate, isononanoic acid ethyl ester and 2-ethyl hexanonic acid ethyl ester, respectively Moesziomyces antarcticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 (R)-2-ethylhexanoic acid ethyl ester + H2O
-
 Moesziomyces antarcticus (R)-2-ethylhexanoate + ethanol
-
 ?
3.1.1.3 (R)-3,5,5-trimethylhexanoic acid ethyl ester + H2O
-
 Moesziomyces antarcticus (R)-3,5,5-trimethylhexanoate + ethanol
-
 ?
3.1.1.3 (S)-2-ethyl hexanoic acid ethyl ester + H2O
-
 Moesziomyces antarcticus ?
-
 ?
3.1.1.3 (S)-3,5,5-trimethylhexanoic acid ethyl ester + H2O
-
 Moesziomyces antarcticus (S)-3,5,5-trimethylhexanoate + ethanol
-
 ?
3.1.1.3 2-ethylhexanoic acid ethyl ester + H2O lowest activity Moesziomyces antarcticus ?
-
 ?
3.1.1.3 diethyl malate + H2O
-
 Moesziomyces antarcticus ?
-
 ?
3.1.1.3 diethyl tartrate + H2O
-
 Moesziomyces antarcticus ?
-
 ?
3.1.1.3 dimethyl succinate + H2O high activity Moesziomyces antarcticus ?
-
 ?
3.1.1.3 ethyl L-lactate + H2O
-
 Moesziomyces antarcticus ethanol + L-lactate
-
 ?
3.1.1.3 isononanoic acid ethyl ester + H2O
-
 Moesziomyces antarcticus isononanoic acid + ethanol
-
 ?
3.1.1.3 L-lysine ethyl ester + H2O
-
 Moesziomyces antarcticus L-lysine + ethanol
-
 ?
3.1.1.3 methyl caprylate + H2O best substrate Moesziomyces antarcticus methanol + caprylate
-
 ?
3.1.1.3 methyl laurate + H2O
-
 Moesziomyces antarcticus methanol + laurate
-
 ?
3.1.1.3 additional information lipase B can only convert straight chain fatty acids of different length with a preference toward C5 and C12 Moesziomyces antarcticus ?
-
 ?
3.1.1.3 triethyl citrate + H2O
-
 Moesziomyces antarcticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 50000, about 50000 Da, SDS-PAGE Moesziomyces antarcticus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase B
-
 Moesziomyces antarcticus
3.1.1.3 Novozyme CALB L commercial preparation Moesziomyces antarcticus