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Literature summary extracted from

  • Chakravarty, A.K.; Shuman, S.
    RNA 3'-phosphate cyclase (RtcA) catalyzes ligase-like adenylylation of DNA and RNA 5'-monophosphate ends (2011), J. Biol. Chem., 286, 4117-4122.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.4 Mg2+ required for activity, 2 mM Mg2+ is used in assay conditions Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.4 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.4 ATP + DNA 5'-terminal-phosphate RtcA also catalyzes adenylylation of 5'-phosphate ends of DNA strands to form AppDNA product Escherichia coli AMP + AppDNA
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate RtcA catalyzes the synthesis of RNA 2',3'-cyclic phosphate ends via an ATP-dependent pathway comprising three nucleotidyl transfer steps: reaction of RtcA with ATP to form a covalent RtcA-(histidinyl-N)-AMP intermediate and release diphosphate, transfer of AMP from RtcA to an RNA 3'-phosphate to form an RNA(3')pp(5')A intermediate, and attack by the terminal nucleoside O2' on the 3'-phosphate to form an RNA 2',3'-cyclic phosphate product and release AMP Escherichia coli AMP + diphosphate + RNA terminal-2',3'-cyclic phosphate
-
 ?
6.5.1.4 ATP + RNA 5'-terminal-phosphate RtcA also catalyzes adenylylation of 5'-phosphate ends of RNA strands to form AppRNA product Escherichia coli AMP + AppRNA
-
 ?
6.5.1.4 additional information RtcA readily adenylylates the 5'-phosphate at a 5'-PO4/3'-OH nick in duplex DNA but is unable to covert the nicked DNA-adenylate to a sealed phosphodiester Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.4 RNA 3'-phosphate cyclase
-
 Escherichia coli
6.5.1.4 RtcA
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.4 5.5 6 pH-optimum for DNA 5'-adenylylation Tris acetate buffer Escherichia coli
6.5.1.4 8 8.5 pH-optimum for RNA 3'-cyclization Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.4 ATP
-
 Escherichia coli