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Literature summary extracted from

  • Pierce, E.; Becker, D.F.; Ragsdale, S.W.
    Identification and characterization of oxalate oxidoreductase, a novel thiamine pyrophosphate-dependent 2-oxoacid oxidoreductase that enables anaerobic growth on oxalate (2010), J. Biol. Chem., 285, 40515-40524.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.10 0.058
-
oxalate in 50 mM Tris-HCl, 2 mM dithiothreitol, pH 7.9, at 25°C Moorella thermoacetica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.10 Fe2+ the enzyme contains 14 mol iron per mol protein Moorella thermoacetica
1.2.7.10 Mg2+ the enzyme contains 0.8 mol Mg2+ per mol protein Moorella thermoacetica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.10 32000
-
1 * 36000 + 1 * 43000 + 1 * 32000, the active protein consists of three peptides in 1:0.8:1 stoichiometry, with estimated sizes of 36000, 43000, and 32000 Da, SDS-PAGE, SDS-PAGE Moorella thermoacetica
1.2.7.10 33900
-
1 * 34200 + 1 * 33900 + 1 * 43700, the active protein consists of three peptides with estimated sizes of 34200, 339000, and 43700 Da, calculated from amino acid sequence Moorella thermoacetica
1.2.7.10 34200
-
1 * 34200 + 1 * 33900 + 1 * 43700, the active protein consists of three peptides with estimated sizes of 34200, 339000, and 43700 Da, calculated from amino acid sequence Moorella thermoacetica
1.2.7.10 36000
-
1 * 36000 + 1 * 43000 + 1 * 32000, the active protein consists of three peptides in 1:0.8:1 stoichiometry, with estimated sizes of 36000, 43000, and 32000 Da, SDS-PAGE, SDS-PAGE Moorella thermoacetica
1.2.7.10 43000
-
1 * 36000 + 1 * 43000 + 1 * 32000, the active protein consists of three peptides in 1:0.8:1 stoichiometry, with estimated sizes of 36000, 43000, and 32000 Da, SDS-PAGE, SDS-PAGE Moorella thermoacetica
1.2.7.10 43700
-
1 * 34200 + 1 * 33900 + 1 * 43700, the active protein consists of three peptides with estimated sizes of 34200, 339000, and 43700 Da, calculated from amino acid sequence Moorella thermoacetica
1.2.7.10 226900
-
analytical ultracentrifugation Moorella thermoacetica
1.2.7.10 236000
-
gel filtration Moorella thermoacetica
1.2.7.10 243000
-
native PAGE Moorella thermoacetica

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.10 Moorella thermoacetica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.10 DEAE-cellulose column chromatography, red agarose column chromatography, phenyl-Sepharose column chromatography, and Q-Sepharose column chromatography Moorella thermoacetica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.7.10 culture condition:glucose-grown cell
-
Moorella thermoacetica
-
1.2.7.10 culture condition:oxalate-grown cell
-
Moorella thermoacetica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.10 0.03
-
enzyme from glucose-grown cells, at pH 7.9 and 55°C Moorella thermoacetica
1.2.7.10 0.4
-
enzyme from oxalate-grown cells, at pH 7.9 and 55°C Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.10 2-oxobutyrate + methyl viologen
-
Moorella thermoacetica ?
-
?
1.2.7.10 2-oxoglutarate + H2O + methyl viologen
-
Moorella thermoacetica ?
-
?
1.2.7.10 2-oxovalerate + H2O + methyl viologen very low activity Moorella thermoacetica ?
-
?
1.2.7.10 glyoxylate + methyl viologen
-
Moorella thermoacetica ?
-
?
1.2.7.10 additional information OOR does not use formate, coenzyme A, ATP, NAD+, and NADP+ and as a substrate Moorella thermoacetica ?
-
?
1.2.7.10 oxalate + cytochrome c cytochrome c is the best electron acceptor Moorella thermoacetica CO2 + reduced cytochrome c
-
?
1.2.7.10 oxalate + FAD
-
Moorella thermoacetica CO2 + FADH2
-
?
1.2.7.10 oxalate + ferredoxin oxalate is the best substrate Moorella thermoacetica CO2 + reduced ferredoxin
-
?
1.2.7.10 oxalate + FMN
-
Moorella thermoacetica CO2 + FMNH2
-
?
1.2.7.10 oxalate + methyl viologen
-
Moorella thermoacetica CO2 + reduced methyl viologen
-
?
1.2.7.10 oxalate + metronidazole
-
Moorella thermoacetica CO2 + reduced metronidazole
-
?
1.2.7.10 oxaloacetate + H2O + methyl viologen
-
Moorella thermoacetica ?
-
?
1.2.7.10 pyruvate + methyl viologen
-
Moorella thermoacetica ?
-
?

Subunits

EC Number Subunits Comment Organism
1.2.7.10 heterotrimer 1 * 34200 + 1 * 33900 + 1 * 43700, the active protein consists of three peptides with estimated sizes of 34200, 339000, and 43700 Da, calculated from amino acid sequence Moorella thermoacetica
1.2.7.10 heterotrimer 1 * 36000 + 1 * 43000 + 1 * 32000, the active protein consists of three peptides in 1:0.8:1 stoichiometry, with estimated sizes of 36000, 43000, and 32000 Da, SDS-PAGE, SDS-PAGE Moorella thermoacetica

Synonyms

EC Number Synonyms Comment Organism
1.2.7.10 OOR
-
Moorella thermoacetica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.7.10 0.09
-
oxalate in 50 mM Tris-HCl, 2 mM dithiothreitol, pH 7.9, at 25°C Moorella thermoacetica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.10 8.7
-
-
Moorella thermoacetica

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.10 4Fe-4S-center the enzyme contains three [Fe4S4] clusters Moorella thermoacetica
1.2.7.10 thiamine diphosphate dependent on Moorella thermoacetica