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Literature summary extracted from

  • Wu, B.; Song, J.; Beitz, E.
    Novel channel enzyme fusion proteins confer arsenate resistance (2010), J. Biol. Chem., 285, 40081-40087.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.20.4.3 expressed in Escherichia coli and yeast Salinispora tropica
1.20.4.4 expressed in Escherichia coli and yeast Salinispora tropica
7.3.2.7 genes strop634 and strop1447, expression of enzyme mutants in Escherichia coli strain EPI300 with pCC1FOS-based fosmids Salinispora tropica

Protein Variants

EC Number Protein Variants Comment Organism
7.3.2.7 additional information construction of a truncated mutant by gene deletions disrupting strop634 and strop1447 in strain CNB-440, lack of Strop634 renders the single and double deletion strains highly sensitive to arsenate exposure, whereas in the absence of Strop1447, the cells unexpectedly display a normal resistance level, complementation of ACR2 deficient yeast strains by expression of Strop634, overview Salinispora tropica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.20.4.4 75
-
arsenate pH 7.5, temperature not specified in the publication Salinispora tropica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.3.2.7 membrane
-
Salinispora tropica 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.20.4.3 43000
-
Western blot Salinispora tropica
1.20.4.4 47000
-
Western blot Salinispora tropica

Organism

EC Number Organism UniProt Comment Textmining
1.20.4.1 Mycobacterium tuberculosis
-
-
-
1.20.4.3 Salinispora tropica
-
-
-
1.20.4.4 Salinispora tropica
-
-
-
7.3.2.7 Frankia alni
-
-
-
7.3.2.7 Mycobacterium tuberculosis
-
gene Rv2643
-
7.3.2.7 Mycobacterium tuberculosis H37Rv
-
gene Rv2643
-
7.3.2.7 Salinispora tropica
-
genes strop634 and strop1447 encoding ACR2 and Ars2, respectively
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.3.2.7 mycelium
-
Salinispora tropica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.4.4 arsenate + thioredoxin
-
Salinispora tropica arsenite + thioredoxin disulfide + H2O
-
?

Subunits

EC Number Subunits Comment Organism
7.3.2.7 More the Strop634-Strop1447 protein interface is compatible permitting heterotetramer formation Salinispora tropica

Synonyms

EC Number Synonyms Comment Organism
1.20.4.1 ACR3-ArsC gene name, protein comprises two domains an aquaglyceroporin-derived N-terminal channel-like part fused to a C-terminal enzyme domain with similarity to ArsC arsenate reductase Mycobacterium tuberculosis
1.20.4.1 arsenate reductase
-
Mycobacterium tuberculosis
1.20.4.3 mycoredoxin
-
Salinispora tropica
1.20.4.3 Strop1447 gene name, protein comprises two domains an aquaglyceroporin-derived N-terminal channel-like part fused to a C-terminal enzyme domain with similarity to ArsC arsenate reductase Salinispora tropica
1.20.4.4 arsenate reductase
-
Salinispora tropica
1.20.4.4 Strop634 gene name, protein comprises two domains an aquaglyceroporin-derived N-terminal channel-like part fused to a C-terminal enzyme domain with similarity to ArsC arsenate reductase, protein also shows minor phosphotyrosine phosphatase activity Salinispora tropica
7.3.2.7 Acr2
-
Salinispora tropica
7.3.2.7 ACR2-ArsC
-
Salinispora tropica
7.3.2.7 ACR3
-
Mycobacterium tuberculosis
7.3.2.7 ACR3
-
Frankia alni
7.3.2.7 ACR3-ArsC
-
Mycobacterium tuberculosis
7.3.2.7 ACR3-ArsC
-
Frankia alni
7.3.2.7 ACR3-ArsC fusion protein
-
Mycobacterium tuberculosis
7.3.2.7 Rv2643
-
Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.20.4.3 7.5
-
assay at Salinispora tropica
1.20.4.4 7.5
-
assay at Salinispora tropica

Cofactor

EC Number Cofactor Comment Organism Structure
1.20.4.3 mycothiol
-
Salinispora tropica
1.20.4.4 thioredoxin
-
Salinispora tropica

General Information

EC Number General Information Comment Organism
1.20.4.1 physiological function as a dual functional protein (arsenite channel and arsenate reductase) ACR3-ArsC rescues a yeast strain that is highly sensitive to arsenate due to deletion of the ACR2 reductase and all transport proteins for arsenite, ACR3, Fps1, and the vacuolar ABC transporter Ycf1 for arsenite-thiol conjugates Mycobacterium tuberculosis
1.20.4.3 malfunction heterologous expression of Strop1447 is not able to rescue a yeast strain lacking arsenate reductase acr2 Salinispora tropica
1.20.4.3 physiological function confers arsenate resistance in Salinispora tropica through a mycothiol-dependent arsenate reduction Salinispora tropica
1.20.4.4 malfunction heterologous expression of Strop634 or its separate arsenate reductase domain complements a yeast strain lacking arsenate reductase acr2 Salinispora tropica
1.20.4.4 malfunction Salinispora tropica Strop634 deletion strains are highly sensitive to arsenate exposure Salinispora tropica
1.20.4.4 physiological function as a dual functional protein (arsenite channel and arsenate reductase) Strop634 rescues a yeast strain that is highly sensitive to arsenate due to deletion of the ACR2 reductase and all transport proteins for arsenite, ACR3, Fps1, and the vacuolar ABC transporter Ycf1 for arsenite-thiol conjugates Salinispora tropica
1.20.4.4 physiological function confers arsenate resistance in Salinispora tropica Salinispora tropica
7.3.2.7 malfunction mutations rendered the aquaglyceroporin channel more polar resulting in lower glycerol permeability and enhanced arsenite selectivity Salinispora tropica
7.3.2.7 additional information the Strop634 channel domain has enhanced selectivity for arsenite and low glycerol permeability Salinispora tropica
7.3.2.7 physiological function the actinomycete from marine environment harbors a fusion protein consisting of an aquaglyceroporin-derived arsenite channel with a C-terminal arsenate reductase domain of phosphotyrosine-phosphatase origin, providing transposable, single gene-encoded arsenate resistance. The arsenate reductase domain couples to thioredoxin and can complement arsenate-sensitive yeast strains. A second isoform Strop1447 with a nonfunctional channel is coupled to the mycothiol/mycoredoxin system and may use the mycothiol/mycoredoxin cofactor pool. The channel-enzyme fusion protein, Strop634, confers arsenate resistance in Salinispora tropica Salinispora tropica
7.3.2.7 physiological function the arctinobacterium from soil harbors a fusion protein consisting of an aquaglyceroporin-derived arsenite channel with a C-terminal arsenate reductase domain of phosphotyrosine-phosphatase origin, providing transposable, single gene-encoded arsenate resistance Frankia alni
7.3.2.7 physiological function the bacterium harbors a fusion protein consisting of an aquaglyceroporin-derived arsenite channel with a C-terminal arsenate reductase domain of phosphotyrosine-phosphatase origin, providing transposable, single gene-encoded arsenate resistance. Strop634 and the ACR3-ArsC fusion protein Rv2643 from Mycobacterium tuberculosis are able to detoxify arsenate, whereas Strop1447 and two chimeras with Strop634 carrying either the Strop1447 channel or ArsC domain are not Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.20.4.4 0.21
-
arsenate pH 7.5, temperature not specified in the publication Salinispora tropica