EC Number | Cloned (Comment) | Organism |
---|---|---|
1.20.4.3 | expressed in Escherichia coli and yeast | Salinispora tropica |
1.20.4.4 | expressed in Escherichia coli and yeast | Salinispora tropica |
7.3.2.7 | genes strop634 and strop1447, expression of enzyme mutants in Escherichia coli strain EPI300 with pCC1FOS-based fosmids | Salinispora tropica |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.3.2.7 | additional information | construction of a truncated mutant by gene deletions disrupting strop634 and strop1447 in strain CNB-440, lack of Strop634 renders the single and double deletion strains highly sensitive to arsenate exposure, whereas in the absence of Strop1447, the cells unexpectedly display a normal resistance level, complementation of ACR2 deficient yeast strains by expression of Strop634, overview | Salinispora tropica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.20.4.4 | 75 | - |
arsenate | pH 7.5, temperature not specified in the publication | Salinispora tropica |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.3.2.7 | membrane | - |
Salinispora tropica | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.20.4.3 | 43000 | - |
Western blot | Salinispora tropica |
1.20.4.4 | 47000 | - |
Western blot | Salinispora tropica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.20.4.1 | Mycobacterium tuberculosis | - |
- |
- |
1.20.4.3 | Salinispora tropica | - |
- |
- |
1.20.4.4 | Salinispora tropica | - |
- |
- |
7.3.2.7 | Frankia alni | - |
- |
- |
7.3.2.7 | Mycobacterium tuberculosis | - |
gene Rv2643 | - |
7.3.2.7 | Mycobacterium tuberculosis H37Rv | - |
gene Rv2643 | - |
7.3.2.7 | Salinispora tropica | - |
genes strop634 and strop1447 encoding ACR2 and Ars2, respectively | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
7.3.2.7 | mycelium | - |
Salinispora tropica | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.20.4.4 | arsenate + thioredoxin | - |
Salinispora tropica | arsenite + thioredoxin disulfide + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.3.2.7 | More | the Strop634-Strop1447 protein interface is compatible permitting heterotetramer formation | Salinispora tropica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.20.4.1 | ACR3-ArsC | gene name, protein comprises two domains an aquaglyceroporin-derived N-terminal channel-like part fused to a C-terminal enzyme domain with similarity to ArsC arsenate reductase | Mycobacterium tuberculosis |
1.20.4.1 | arsenate reductase | - |
Mycobacterium tuberculosis |
1.20.4.3 | mycoredoxin | - |
Salinispora tropica |
1.20.4.3 | Strop1447 | gene name, protein comprises two domains an aquaglyceroporin-derived N-terminal channel-like part fused to a C-terminal enzyme domain with similarity to ArsC arsenate reductase | Salinispora tropica |
1.20.4.4 | arsenate reductase | - |
Salinispora tropica |
1.20.4.4 | Strop634 | gene name, protein comprises two domains an aquaglyceroporin-derived N-terminal channel-like part fused to a C-terminal enzyme domain with similarity to ArsC arsenate reductase, protein also shows minor phosphotyrosine phosphatase activity | Salinispora tropica |
7.3.2.7 | Acr2 | - |
Salinispora tropica |
7.3.2.7 | ACR2-ArsC | - |
Salinispora tropica |
7.3.2.7 | ACR3 | - |
Mycobacterium tuberculosis |
7.3.2.7 | ACR3 | - |
Frankia alni |
7.3.2.7 | ACR3-ArsC | - |
Mycobacterium tuberculosis |
7.3.2.7 | ACR3-ArsC | - |
Frankia alni |
7.3.2.7 | ACR3-ArsC fusion protein | - |
Mycobacterium tuberculosis |
7.3.2.7 | Rv2643 | - |
Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.20.4.3 | 7.5 | - |
assay at | Salinispora tropica |
1.20.4.4 | 7.5 | - |
assay at | Salinispora tropica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.20.4.3 | mycothiol | - |
Salinispora tropica | |
1.20.4.4 | thioredoxin | - |
Salinispora tropica |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.20.4.1 | physiological function | as a dual functional protein (arsenite channel and arsenate reductase) ACR3-ArsC rescues a yeast strain that is highly sensitive to arsenate due to deletion of the ACR2 reductase and all transport proteins for arsenite, ACR3, Fps1, and the vacuolar ABC transporter Ycf1 for arsenite-thiol conjugates | Mycobacterium tuberculosis |
1.20.4.3 | malfunction | heterologous expression of Strop1447 is not able to rescue a yeast strain lacking arsenate reductase acr2 | Salinispora tropica |
1.20.4.3 | physiological function | confers arsenate resistance in Salinispora tropica through a mycothiol-dependent arsenate reduction | Salinispora tropica |
1.20.4.4 | malfunction | heterologous expression of Strop634 or its separate arsenate reductase domain complements a yeast strain lacking arsenate reductase acr2 | Salinispora tropica |
1.20.4.4 | malfunction | Salinispora tropica Strop634 deletion strains are highly sensitive to arsenate exposure | Salinispora tropica |
1.20.4.4 | physiological function | as a dual functional protein (arsenite channel and arsenate reductase) Strop634 rescues a yeast strain that is highly sensitive to arsenate due to deletion of the ACR2 reductase and all transport proteins for arsenite, ACR3, Fps1, and the vacuolar ABC transporter Ycf1 for arsenite-thiol conjugates | Salinispora tropica |
1.20.4.4 | physiological function | confers arsenate resistance in Salinispora tropica | Salinispora tropica |
7.3.2.7 | malfunction | mutations rendered the aquaglyceroporin channel more polar resulting in lower glycerol permeability and enhanced arsenite selectivity | Salinispora tropica |
7.3.2.7 | additional information | the Strop634 channel domain has enhanced selectivity for arsenite and low glycerol permeability | Salinispora tropica |
7.3.2.7 | physiological function | the actinomycete from marine environment harbors a fusion protein consisting of an aquaglyceroporin-derived arsenite channel with a C-terminal arsenate reductase domain of phosphotyrosine-phosphatase origin, providing transposable, single gene-encoded arsenate resistance. The arsenate reductase domain couples to thioredoxin and can complement arsenate-sensitive yeast strains. A second isoform Strop1447 with a nonfunctional channel is coupled to the mycothiol/mycoredoxin system and may use the mycothiol/mycoredoxin cofactor pool. The channel-enzyme fusion protein, Strop634, confers arsenate resistance in Salinispora tropica | Salinispora tropica |
7.3.2.7 | physiological function | the arctinobacterium from soil harbors a fusion protein consisting of an aquaglyceroporin-derived arsenite channel with a C-terminal arsenate reductase domain of phosphotyrosine-phosphatase origin, providing transposable, single gene-encoded arsenate resistance | Frankia alni |
7.3.2.7 | physiological function | the bacterium harbors a fusion protein consisting of an aquaglyceroporin-derived arsenite channel with a C-terminal arsenate reductase domain of phosphotyrosine-phosphatase origin, providing transposable, single gene-encoded arsenate resistance. Strop634 and the ACR3-ArsC fusion protein Rv2643 from Mycobacterium tuberculosis are able to detoxify arsenate, whereas Strop1447 and two chimeras with Strop634 carrying either the Strop1447 channel or ArsC domain are not | Mycobacterium tuberculosis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.20.4.4 | 0.21 | - |
arsenate | pH 7.5, temperature not specified in the publication | Salinispora tropica |