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Literature summary extracted from

  • Nogales, J.; Canales, A.; Jimenez-Barbero, J.; Garcia, J.L.; Diaz, E.
    Molecular characterization of the gallate dioxygenase from Pseudomonas putida KT2440. The prototype of a new subgroup of extradiol dioxygenases (2005), J. Biol. Chem., 280, 35382-35390.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.57 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.57 2,2'-dipyridyl 2 mM 2,2'-dipyridyl, a specific Fe2+-chelator, abolishes the gallate dioxygenase activity completely Pseudomonas putida
1.13.11.57 EDTA complete inhibition at 1 mM, the activity is only fully recovered with Fe2+ Pseudomonas putida
1.13.11.57 H2O2 hydrogen peroxide at 1 mM reduces the gallate dioxygenase activity to 15%, and the activity can be partially restored (50% activity of the untreated enzyme) upon addition of 10 mM ascorbate Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.57 0.144
-
 gallate in 100 mM Tris-HCl, pH 7.0, at 30°C Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.57 Fe2+ required for activity Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.57 47000
-
 3 * 47000, SDS-PAGE Pseudomonas putida
1.13.11.57 47644
-
 3 * 47644, calculated from amino acid sequence Pseudomonas putida
1.13.11.57 132000
-
 molar mass obtained by sedimentation equilibrium Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.57 Pseudomonas putida
-
-
-
1.13.11.57 Pseudomonas putida KT 2240
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.57 ultracentrifugation and phenyl-Sepharose column chromatography Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.57 21.4
-
 crude extract, in 100 mM Tris-HCl, pH 7.0, at 30°C Pseudomonas putida
1.13.11.57 42.7
-
 after 2fold purification, in 100 mM Tris-HCl, pH 7.0, at 30°C Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.57 gallate + O2 the substrate specificity is very restricted toward gallate Pseudomonas putida 4-oxalomesaconate
-
 ?
1.13.11.57 gallate + O2 the substrate specificity is very restricted toward gallate Pseudomonas putida KT 2240 4-oxalomesaconate
-
 ?
1.13.11.57 additional information no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate Pseudomonas putida ?
-
 ?
1.13.11.57 additional information no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate Pseudomonas putida KT 2240 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.57 homotrimer 3 * 47000, SDS-PAGE Pseudomonas putida
1.13.11.57 homotrimer 3 * 47644, calculated from amino acid sequence Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.13.11.57 GALA
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.57 7
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.57 6 9
-
 Pseudomonas putida

Expression

EC Number Organism Comment Expression
1.13.11.57 Pseudomonas putida the expression of the galA gene is specifically induced by gallate up

General Information

EC Number General Information Comment Organism
1.13.11.57 physiological function the GalA protein is the only dioxygenase involved in gallate cleavage in Pseudomonas putida KT2440 Pseudomonas putida