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Literature summary extracted from

  • Schwientek, T.; Almeida, R.M., Levery, S.B.; Holmes, E.H.; Bennett, E.; Clausen, H.
    Cloning of a novel member of the UDP-galactose:beta-N-acetylglucosamine beta1,4-galactosyltransferase family, beta4Gal-T4, involved in glycosphingolipid biosynthesis (1998), J. Biol. Chem., 273, 29331-29340.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.275 alpha-lactalbumin the enzyme shows a marked increase in N-acetyllactosamine synthase activity in the presence of alpha-lactalbumin. Two-fold activation is achieved at 0.25 mg/ml and almost 8fold at 20 mg/ml Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.275 expression in insect cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.275 additional information the enzyme is not inhibited at high concentrations of either benzyl-beta-D-N-acetyl-D-glucosamine or free N-acetylglucosamine Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.275 UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide Homo sapiens
-
 UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.275 Homo sapiens O60513
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.275 brain
-
 Homo sapiens
-
2.4.1.275 central nervous system
-
 Homo sapiens
-
2.4.1.275 colon
-
 Homo sapiens
-
2.4.1.275 heart
-
 Homo sapiens
-
2.4.1.275 lung
-
 Homo sapiens
-
2.4.1.275 muscle
-
 Homo sapiens
-
2.4.1.275 ovary
-
 Homo sapiens
-
2.4.1.275 placenta
-
 Homo sapiens
-
2.4.1.275 testis
-
 Homo sapiens
-
2.4.1.275 uterus
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.275 additional information free glucose is not an acceptor for the enzyme. The enzyme does not transfer galactose to asialo-agalacto-fetuin, asialo-agalactotransferrin, or ovalbumin Homo sapiens ?
-
 ?
2.4.1.275 UDP-alpha-D-galactose + 1-O-benzyl 6-O-beta-D-GlcpNAC-beta-D-GlypNAc
-
 Homo sapiens UDP + 1-O-benzyl 4-O-beta-D-Galp-6-O-beta-D-GlcpNAC-beta-D-GlcpNAc
-
 ?
2.4.1.275 UDP-alpha-D-galactose + 1-O-benzyl beta-D-GlcpNAc no activity with alpha-D-GlcNAc-1-benzyl Homo sapiens UDP + 1-O-benzyl 4-O-beta-D-Galp-beta-D-GlcpNAc
-
 ?
2.4.1.275 UDP-alpha-D-galactose + 1-O-methyl 3-O-beta-D-GlcpNAc-beta-D-Galp
-
 Homo sapiens UDP + 1-O-methyl 4-O-beta-D-Galp-3-O-beta-D-GlcpNAc-beta-D-Galp
-
 ?
2.4.1.275 UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 Homo sapiens UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide
-
 ?
2.4.1.275 UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->)-ceramide i.e. lactotriaosylceramide. beta4Gal-T4 shows strict donor substrate specificity for UDP-Gal and did not utilize UDP-GalNAc or UDP-GlcNAc with the acceptor substrates tested Homo sapiens UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide
-
 ?
2.4.1.275 UDP-galactose + 1-O-(1-methylumbellifer-7-yl) beta-D-GlcpNAc
-
 Homo sapiens UDP + 1-O-(1-methylumbellifer-7-yl) 4-O-beta-D-Galp-beta-D-GlcpNAc
-
 ?
2.4.1.275 UDP-galactose + 1-O-(4-nitrophenyl) beta-D-GlcpNAc
-
 Homo sapiens UDP + 1-O-(4-nitrophenyl) 4-O-beta-D-Galp-beta-D-GlcpNAc
-
 ?
2.4.1.275 UDP-galactose + 1-O-methyl 6-O-beta-D-GlcpNAC-alpha-D-Manp
-
 Homo sapiens UDP + 1-O-methyl 4-O-beta-D-Galp-6-O-beta-D-GlcpNAC-alpha-D-Manp
-
 ?
2.4.1.275 UDP-galactose + 1-S-(4-nitrophenyl) beta-D-1-thio-GlcpNAc
-
 Homo sapiens UDP + 1-S-(4-nitrophenyl) 4-O-beta-D-Galp-beta-D-1-thio-GlcpNAc
-
 ?
2.4.1.275 UDP-galactose + beta-D-GlcpNAc-(1->6)-D-GlcpNAc
-
 Homo sapiens UDP + beta-D-Galp-(1->4)-[beta-D-GlcpNAc-(1->6)]-D-GlcpNAc
-
 ?
2.4.1.275 UDP-galactose + N,N',N''-triacetylchitotriose
-
 Homo sapiens UDP + beta-D-Galp-(1->4)-beta-D-GlcpNAc-(1->4)-beta-D-GlcpNAc-(1->4)-D-GlcpNAc
-
 ?
2.4.1.275 UDP-galactose + N,N'-diacetylchitobiose
-
 Homo sapiens UDP + beta-D-Galp-(1->4)-beta-D-GlcpNAc-(1->4)-D-GlcpNAc
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.275 beta4Gal-T4
-
 Homo sapiens
2.4.1.275 UDP-galactose:beta-N-acetylglucosamine beta1,4-galactosyltransferase
-
 Homo sapiens
2.4.1.275 UDPGal:betaGlcNAc beta1,4Gal-transferase
-
 Homo sapiens