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Literature summary extracted from

  • Ito, R.; Sekiguchi, M.; Setoyama, D.; Nakatsu, Y.; Yamagata, Y.; Hayakawa, H.
    Cleavage of oxidized guanine nucleotide and ADP sugar by human NUDT5 protein (2011), J. Biochem., 149, 731-738.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.13 expressed in Escherichia coli M15 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.13 8-oxo-dGDP the ADP-ribose cleavage is competitively inhibited by 8-oxo-dGDP (68% relative cleavage efficiency at 0.01 mM) Homo sapiens
3.6.1.13 8-oxo-dGMP the ADP-ribose cleavage is competitively inhibited by 8-oxo-dGMP (89% relative cleavage efficiency at 0.01 mM) Homo sapiens
3.6.1.13 ADP-ribose the 8-oxo-dGDP cleavage is competitively inhibited by ADP-ribose (22% relative cleavage efficiency at 0.005 mM) Homo sapiens
3.6.1.13 AMP the 8-oxo-dGDP cleavage is competitively inhibited by AMP (30% relative cleavage efficiency at 0.005 mM) Homo sapiens
3.6.1.13 additional information no inhibitory effect is observed with 8-oxo-dGMP Homo sapiens
3.6.1.58 8-oxo-dGDP inhibits cleavage of ADP-ribose Homo sapiens
3.6.1.58 ADP-ribose inhibits cleavage of 8-oxo-dGTP Homo sapiens
3.6.1.58 AMP inhibits cleavage of 8-oxo-dGTP Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.13 0.0019
-
 ADP-ribose at pH 8.0 and 37°C Homo sapiens
3.6.1.13 0.0035
-
 8-oxo-dGDP at pH 10.0 and 37°C Homo sapiens
3.6.1.13 0.0038
-
 8-oxo-dGDP at pH 8.0 and 37°C Homo sapiens
3.6.1.13 0.036
-
 ADP-ribose at pH 10.0 and 37°C Homo sapiens
3.6.1.58 0.0035
-
 8-oxo-dGDP pH 10.0, 30°C Homo sapiens
3.6.1.58 0.0038
-
 8-oxo-dGDP pH 8, 30°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.13 Homo sapiens Q9UKK9
-
-
3.6.1.58 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.13 Talon resin column chromatography Homo sapiens
3.6.1.58
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.13 8-oxo-dGDP + H2O
-
 Homo sapiens 8-oxo-dGMP + phosphate
-
 ?
3.6.1.13 8-oxo-dGTP + H2O the enzyme hardly acts on 8-oxo-dGTP Homo sapiens ?
-
 ?
3.6.1.13 ADP-ribose + H2O ADP-ribose is a better substrate compared with 8-oxo-dGDP Homo sapiens AMP + ribose 5-phosphate
-
 ?
3.6.1.58 8-oxo-dGDP + H2O ADP-ribose is a better substrate for NUDT5 compared with oxidized guanine nucleotides Homo sapiens 8-oxo-dGMP + phosphate
-
 ?
3.6.1.58 additional information the bifunctional enzyme also hydrolyses ADP-ribose to ADP-ribose and ribose 5-phosphate (cf. EC 3.6.1.13) Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.13 NUDT5
-
 Homo sapiens
3.6.1.58 NUDT5
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.58 30
-
 hydrolysis of 8-oxo-dGDP, assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.13 7 9 for cleavage of ADP-ribose Homo sapiens
3.6.1.13 10
-
 for cleavage of 8-oxo-dGDP Homo sapiens
3.6.1.58 10
-
 cleavage of 8-oxo-dGDP Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.58 9.5 12 pH 9.5: about 50% of maximal activity, pH 12.0: about 50% of maximal activity Homo sapiens