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Literature summary extracted from

  • Mogi, T.; Ano, Y.; Nakatsuka, T.; Toyama, H.; Muroi, A.; Miyoshi, H.; Migita, C.T.; Ui, H.; Shiomi, K.; Omura, S.; Kita, K.; Matsushita, K.
    Biochemical and spectroscopic properties of cyanide-insensitive quinol oxidase from Gluconobacter oxydans (2009), J. Biochem., 146, 263-271.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.11 2-Heptyl-4-hydroxyquinoline-N-oxide
-
Gluconobacter oxydans
1.10.3.11 antimycin A 56% residual activity at 0.005 mg/ml; 56% residual activity at 0.005 mg/ml Gluconobacter oxydans
1.10.3.11 aurachin C 1-10 15% residual activity at 0.005 mg/ml; 15% residual activity at 0.005 mg/ml Gluconobacter oxydans
1.10.3.11 aurachin D 16% residual activity at 0.005 mg/ml; 16% residual activity at 0.005 mg/ml Gluconobacter oxydans
1.10.3.11 cyanide quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration; quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration Gluconobacter oxydans
1.10.3.11 Gramicidin S 67% residual activity at 0.005 mg/ml; 67% residual activity at 0.005 mg/ml Gluconobacter oxydans
1.10.3.11 LL-Z1272gamma 69% residual activity at 0.005 mg/ml; 69% residual activity at 0.005 mg/ml Gluconobacter oxydans
1.10.3.11 additional information not inhibited by ascofuranone; not inhibited by ascofuranone Gluconobacter oxydans
1.10.3.11 Piericidin A 14% residual activity at 0.005 mg/ml; 14% residual activity at 0.005 mg/ml Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.11 0.0403
-
ubiquinol-1 at 25°C in 50 mM potassium phosphate (pH 6.5) Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.11 membrane
-
Gluconobacter oxydans 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.11 37813
-
1 * 53583 + 1 * 37813, calculated from amino acid sequence Gluconobacter oxydans
1.10.3.11 44000
-
1 * 54000 + 1 * 44000, SDS-PAGE Gluconobacter oxydans
1.10.3.11 53583
-
1 * 53583 + 1 * 37813, calculated from amino acid sequence Gluconobacter oxydans
1.10.3.11 54000
-
1 * 54000 + 1 * 44000, SDS-PAGE Gluconobacter oxydans

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.11 Gluconobacter oxydans
-
subunit I; formerly Gluconobacter suboxydans strain IFO12528
-
1.10.3.11 Gluconobacter oxydans Q5FU84 subunit II; formerly Gluconobacter suboxydans strain IFO12528. The enzyme from strain 621H is expressed in strain NBRC3172
-
1.10.3.11 Gluconobacter oxydans 621H Q5FU84 subunit II; formerly Gluconobacter suboxydans strain IFO12528. The enzyme from strain 621H is expressed in strain NBRC3172
-
1.10.3.11 Gluconobacter oxydans NBRC 3172
-
subunit I; formerly Gluconobacter suboxydans strain IFO12528
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.11 ubiquinol-1 + O2
-
Gluconobacter oxydans ubiquinone-1 + H2O
-
?
1.10.3.11 ubiquinol-1 + O2
-
Gluconobacter oxydans 621H ubiquinone-1 + H2O
-
?
1.10.3.11 ubiquinol-1 + O2
-
Gluconobacter oxydans NBRC 3172 ubiquinone-1 + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.10.3.11 heterodimer 1 * 53583 + 1 * 37813, calculated from amino acid sequence Gluconobacter oxydans
1.10.3.11 heterodimer 1 * 54000 + 1 * 44000, SDS-PAGE Gluconobacter oxydans

Synonyms

EC Number Synonyms Comment Organism
1.10.3.11 CIO
-
Gluconobacter oxydans
1.10.3.11 CioAB
-
Gluconobacter oxydans
1.10.3.11 cyanide-insensitive oxidase
-
Gluconobacter oxydans
1.10.3.11 cyanide-insensitive quinol oxidase
-
Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.10.3.11 heme heme b558, heme b595 and heme d Gluconobacter oxydans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.10.3.11 0.00004
-
at 25°C in 50 mM potassium phosphate (pH 6.5) Gluconobacter oxydans aurachin C 1-10
1.10.3.11 0.0007
-
at 25°C in 50 mM potassium phosphate (pH 6.5) Gluconobacter oxydans Piericidin A
1.10.3.11 0.008
-
quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration with the IC50 of 0.008 mM (relative amplitude, 21%) and 0.013 mM (79%), at 25°C in 50 mM potassium phosphate (pH 6.5) Gluconobacter oxydans cyanide
1.10.3.11 0.013
-
at 25°C in 50 mM potassium phosphate (pH 6.5) Gluconobacter oxydans 2-Heptyl-4-hydroxyquinoline-N-oxide
1.10.3.11 0.013
-
at 25°C in 50 mM potassium phosphate (pH 6.5) Gluconobacter oxydans LL-Z1272gamma
1.10.3.11 0.017
-
at 25°C in 50 mM potassium phosphate (pH 6.5) Gluconobacter oxydans antimycin A
1.10.3.11 0.04
-
at 25°C in 50 mM potassium phosphate (pH 6.5) Gluconobacter oxydans Gramicidin S
1.10.3.11 13
-
quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration with the IC50 of 0.008 mM (relative amplitude, 21%) and 13 mM (79%), at 25°C in 50 mM potassium phosphate (pH 6.5) Gluconobacter oxydans cyanide