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Literature summary extracted from

  • Mosberg, J.A.; Yep, A.; Meredith, T.C.; Smith, S.; Wang, P.F.; Holler, T.P.; Mobley, H.L.; Woodard, R.W.
    A unique arabinose 5-phosphate isomerase found within a genomic island associated with the uropathogenicity of Escherichia coli CFT073 (2011), J. Bacteriol., 193, 2981-2988.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.1.13 EDTA EDTA treatment increases the activity of c3406 protein by approximately 2.5fold Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.13 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.13 Cd2+
-
Escherichia coli
5.3.1.13 Hg2+
-
Escherichia coli
5.3.1.13 Zn2+
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.13 0.7
-
D-ribulose 5-phosphate pH 6.6, 37°C Escherichia coli
5.3.1.13 1.92
-
D-arabinose 5-phosphate pH 6.6, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.13 Ba2+ stimulates activity Escherichia coli
5.3.1.13 Ca2+ stimulates activity Escherichia coli
5.3.1.13 Co2+ stimulates activity Escherichia coli
5.3.1.13 Cu2+ stimulates activity Escherichia coli
5.3.1.13 Fe2+ stimulates activity Escherichia coli
5.3.1.13 Mg2+ stimulates activity Escherichia coli
5.3.1.13 Mn2+ stimulates activity Escherichia coli
5.3.1.13 Ni2+ stimulates activity Escherichia coli
5.3.1.13 Zn2+ c3406 protein possesses 0.72 equivalent of Zn2+ per subunit Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.13 20888
-
x * 20888, mass spectrometry Escherichia coli
5.3.1.13 21000
-
x * 21000, SDS-PAGE Escherichia coli
5.3.1.13 73000
-
gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.13 Escherichia coli
-
-
-
5.3.1.13 Escherichia coli CFT073
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.13 ammonium sulfate precipitation and MonoQ column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.13 D-Arabinose 5-phosphate
-
Escherichia coli D-Ribulose 5-phosphate
-
r
5.3.1.13 D-Arabinose 5-phosphate
-
Escherichia coli CFT073 D-Ribulose 5-phosphate
-
r
5.3.1.13 D-ribulose 5-phosphate
-
Escherichia coli D-arabinose 5-phosphate
-
r
5.3.1.13 D-ribulose 5-phosphate
-
Escherichia coli CFT073 D-arabinose 5-phosphate
-
r
5.3.1.13 additional information D-ribose 5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose 6-phosphate, and D-glucosamine 6-phosphate are no substrates Escherichia coli ?
-
?
5.3.1.13 additional information D-ribose 5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose 6-phosphate, and D-glucosamine 6-phosphate are no substrates Escherichia coli CFT073 ?
-
?

Subunits

EC Number Subunits Comment Organism
5.3.1.13 trimer or tetramer x * 21000, SDS-PAGE Escherichia coli
5.3.1.13 trimer or tetramer x * 20888, mass spectrometry Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
5.3.1.13 A5P isomerase
-
Escherichia coli
5.3.1.13 arabinose 5-phosphate isomerase
-
Escherichia coli
5.3.1.13 c3406 protein
-
Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.13 10.5
-
D-ribulose 5-phosphate pH 6.6, 37°C Escherichia coli
5.3.1.13 16.8
-
D-arabinose 5-phosphate pH 6.6, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.13 6.6
-
-
Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.13 5.5 7.5 only trace A5P isomerase activity observed below pH 5.5 or above pH 7.5 Escherichia coli

General Information

EC Number General Information Comment Organism
5.3.1.13 malfunction a c3406 deletion mutant is still able to compete with wild type CFT073 in a transurethral cochallenge in mice and cannot colonize the mouse kidney Escherichia coli
5.3.1.13 physiological function the presence of c3406 is not essential for a pathogenic phenotype Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.13 8.8
-
D-arabinose 5-phosphate pH 6.6, 37°C Escherichia coli
5.3.1.13 15
-
D-ribulose 5-phosphate pH 6.6, 37°C Escherichia coli