EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.13 | EDTA | EDTA treatment increases the activity of c3406 protein by approximately 2.5fold | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.13 | expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.13 | Cd2+ | - |
Escherichia coli | |
5.3.1.13 | Hg2+ | - |
Escherichia coli | |
5.3.1.13 | Zn2+ | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.13 | 0.7 | - |
D-ribulose 5-phosphate | pH 6.6, 37°C | Escherichia coli | |
5.3.1.13 | 1.92 | - |
D-arabinose 5-phosphate | pH 6.6, 37°C | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.13 | Ba2+ | stimulates activity | Escherichia coli | |
5.3.1.13 | Ca2+ | stimulates activity | Escherichia coli | |
5.3.1.13 | Co2+ | stimulates activity | Escherichia coli | |
5.3.1.13 | Cu2+ | stimulates activity | Escherichia coli | |
5.3.1.13 | Fe2+ | stimulates activity | Escherichia coli | |
5.3.1.13 | Mg2+ | stimulates activity | Escherichia coli | |
5.3.1.13 | Mn2+ | stimulates activity | Escherichia coli | |
5.3.1.13 | Ni2+ | stimulates activity | Escherichia coli | |
5.3.1.13 | Zn2+ | c3406 protein possesses 0.72 equivalent of Zn2+ per subunit | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.1.13 | 20888 | - |
x * 20888, mass spectrometry | Escherichia coli |
5.3.1.13 | 21000 | - |
x * 21000, SDS-PAGE | Escherichia coli |
5.3.1.13 | 73000 | - |
gel filtration | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.13 | Escherichia coli | - |
- |
- |
5.3.1.13 | Escherichia coli CFT073 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.13 | ammonium sulfate precipitation and MonoQ column chromatography | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.13 | D-Arabinose 5-phosphate | - |
Escherichia coli | D-Ribulose 5-phosphate | - |
r | |
5.3.1.13 | D-Arabinose 5-phosphate | - |
Escherichia coli CFT073 | D-Ribulose 5-phosphate | - |
r | |
5.3.1.13 | D-ribulose 5-phosphate | - |
Escherichia coli | D-arabinose 5-phosphate | - |
r | |
5.3.1.13 | D-ribulose 5-phosphate | - |
Escherichia coli CFT073 | D-arabinose 5-phosphate | - |
r | |
5.3.1.13 | additional information | D-ribose 5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose 6-phosphate, and D-glucosamine 6-phosphate are no substrates | Escherichia coli | ? | - |
? | |
5.3.1.13 | additional information | D-ribose 5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose 6-phosphate, and D-glucosamine 6-phosphate are no substrates | Escherichia coli CFT073 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.13 | trimer or tetramer | x * 21000, SDS-PAGE | Escherichia coli |
5.3.1.13 | trimer or tetramer | x * 20888, mass spectrometry | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.13 | A5P isomerase | - |
Escherichia coli |
5.3.1.13 | arabinose 5-phosphate isomerase | - |
Escherichia coli |
5.3.1.13 | c3406 protein | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.13 | 10.5 | - |
D-ribulose 5-phosphate | pH 6.6, 37°C | Escherichia coli | |
5.3.1.13 | 16.8 | - |
D-arabinose 5-phosphate | pH 6.6, 37°C | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.13 | 6.6 | - |
- |
Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.13 | 5.5 | 7.5 | only trace A5P isomerase activity observed below pH 5.5 or above pH 7.5 | Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.3.1.13 | malfunction | a c3406 deletion mutant is still able to compete with wild type CFT073 in a transurethral cochallenge in mice and cannot colonize the mouse kidney | Escherichia coli |
5.3.1.13 | physiological function | the presence of c3406 is not essential for a pathogenic phenotype | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.13 | 8.8 | - |
D-arabinose 5-phosphate | pH 6.6, 37°C | Escherichia coli | |
5.3.1.13 | 15 | - |
D-ribulose 5-phosphate | pH 6.6, 37°C | Escherichia coli |