EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.1.2 | A305G | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 16% of wild-type activity with L-glutamate, 2% of wild-type activity with L-glutamine | Bacillus subtilis |
6.3.1.2 | E304D | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 37% of wild-type activity with L-glutamate, 8% of wild-type activity with L-glutamine | Bacillus subtilis |
6.3.1.2 | E304H | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 43% of wild-type activity with L-glutamate, 5% of wild-type activity with L-glutamine | Bacillus subtilis |
6.3.1.2 | G302A | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 80% of wild-type activity with L-glutamate, 58% of wild-type activity with L-glutamine | Bacillus subtilis |
6.3.1.2 | P306A | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 160% of wild-type activity with L-glutamate, 130% of wild-type activity with L-glutamine | Bacillus subtilis |
6.3.1.2 | V300A | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 76% of wild-type activity with L-glutamate, 18% of wild-type activity with L-glutamine | Bacillus subtilis |
6.3.1.2 | Y303A | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 36% of wild-type activity with L-glutamate, 8% of wild-type activity with L-glutamine | Bacillus subtilis |
6.3.1.2 | Y303H | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 150% of wild-type activity with L-glutamate, 100% of wild-type activity with L-glutamine | Bacillus subtilis |
6.3.1.2 | Y303L | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 87% of wild-type activity with L-glutamate, 46% of wild-type activity with L-glutamine | Bacillus subtilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | AMP | - |
Bacillus subtilis | |
6.3.1.2 | glycine | - |
Bacillus subtilis | |
6.3.1.2 | L-glutamine | feedback inhibition | Bacillus subtilis | |
6.3.1.2 | L-methionine-S-sulfoximine | - |
Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.2 | 0.18 | - |
NH3 | wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 0.68 | - |
hydroxylamine | mutant E304H, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 0.74 | - |
hydroxylamine | mutant E304D, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 0.83 | - |
hydroxylamine | wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 1.2 | - |
ATP | mutant E304H, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 2.4 | - |
ATP | wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 3.4 | - |
L-glutamate | mutant E304H, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 5.3 | - |
ATP | mutant G302A, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 9.4 | - |
ATP | mutant Y303H, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 10 | - |
L-glutamate | mutant E304D, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 11 | - |
ATP | mutant E304D, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 27 | - |
L-glutamate | wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 32 | - |
NH3 | mutant E304H, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 59 | - |
L-glutamate | mutant Y303H, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 84 | - |
L-glutamate | mutant G302A, 25°C, pH not specified in the publication | Bacillus subtilis | |
6.3.1.2 | 120 | - |
NH3 | mutant E304D, 25°C, pH not specified in the publication | Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | Mg2+ | required | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.1.2 | Bacillus subtilis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.2 | ATP + L-glutamate + NH3 | - |
Bacillus subtilis | ADP + phosphate + L-glutamine | - |
? | |
6.3.1.2 | L-glutamine + hydroxylamine + ADP | partial reverse reaction | Bacillus subtilis | gamma-glutamylhydroxamate + NH3 + ? | - |
? |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
6.3.1.2 | 0.1 | - |
mutant Y303H, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
6.3.1.2 | 0.1 | - |
mutant Y303L, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
6.3.1.2 | 0.12 | - |
mutant V300A, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
6.3.1.2 | 0.13 | - |
wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
6.3.1.2 | 0.2 | - |
mutant P306A, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
6.3.1.2 | 0.25 | - |
mutant P306A, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
6.3.1.2 | 0.28 | - |
mutant G302A, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
6.3.1.2 | 0.5 | - |
wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
6.3.1.2 | 0.61 | - |
mutant Y303A, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
6.3.1.2 | 1 | - |
mutant Y303H, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
6.3.1.2 | 1.3 | - |
mutant Y303A, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
6.3.1.2 | 1.4 | - |
mutant V300A, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
6.3.1.2 | 1.5 | - |
mutant P306A, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
6.3.1.2 | 1.5 | - |
mutant Y303L, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
6.3.1.2 | 1.7 | - |
mutant E304D, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
6.3.1.2 | 1.7 | - |
mutant G302A, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
6.3.1.2 | 2.1 | - |
mutant A305G, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
6.3.1.2 | 2.4 | - |
wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
6.3.1.2 | 2.7 | - |
mutant A305G, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
6.3.1.2 | 4.1 | - |
mutant Y303H, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
6.3.1.2 | 7 | - |
mutant V300A, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
6.3.1.2 | 11 | - |
mutant G302A, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
6.3.1.2 | 13 | - |
mutant Y303A, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
6.3.1.2 | 19 | - |
mutant Y303L, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
6.3.1.2 | 20 | - |
mutant E304D, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
6.3.1.2 | 26 | - |
mutant E304A, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
6.3.1.2 | 30 | - |
mutant E304A, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
6.3.1.2 | 31 | - |
wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | glycine | |
6.3.1.2 | 79 | - |
mutant E304D, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
6.3.1.2 | 140 | - |
mutant A305G, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
6.3.1.2 | 140 | - |
mutant E304A, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
6.3.1.2 | 350 | - |
mutant A305G, 25°C, pH not specified in the publication | Bacillus subtilis | glycine |