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Literature summary extracted from

  • Wang, W.; Li, J.; Wang, K.; Smirnova, T.I.; Oldfield, E.
    Pyridine inhibitor binding to the 4Fe-4S protein A. aeolicus IspH (LytB): a HYSCORE Investigation (2011), J. Am. Chem. Soc., 133, 6525-6528.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.7.4 2-(pyridin-3-yl)ethyl diphosphate i.e. BPH-1027 Aquifex aeolicus
1.17.7.4 BPH-1029
-
 Aquifex aeolicus
1.17.7.4 BPH-293
-
 Aquifex aeolicus
1.17.7.4 Imidazol
-
 Aquifex aeolicus
1.17.7.4 additional information pyridine inhibitors of IspH directly bind to the unique fourth Fe in the 4Fe-4S cluster, spectral binding structure analysis and inhibitor design, overview Aquifex aeolicus
1.17.7.4 N-methylpyridinium
-
 Aquifex aeolicus
1.17.7.4 pyridin-2-ylmethyl diphosphate i.e. BPH-1029 Aquifex aeolicus
1.17.7.4 pyridin-3-ylmethyl diphosphate i.e. BPH-293 Aquifex aeolicus
1.17.7.4 pyridin-4-ylmethyl trihydrogen diphosphate i.e. BPH-1030 Aquifex aeolicus
1.17.7.4 pyridine-N-oxide
-
 Aquifex aeolicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.7.4 Fe2+ IspH is a 4Fe-4S protein Aquifex aeolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ Aquifex aeolicus formation of a mixture of isopentenyl diphosphate and dimethylallyl diphosphate in a ratio 1:5 dimethylallyl diphosphate + NAD(P)+ + H2O
-
 r
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ Aquifex aeolicus formation of a mixture of isopentenyl diphosphate and dimethylallyl diphosphate in a ratio 1:5 isopentenyl diphosphate + NAD(P)+ + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.17.7.4 Aquifex aeolicus
-
 gene ispH or lytB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ formation of a mixture of isopentenyl diphosphate and dimethylallyl diphosphate in a ratio 1:5 Aquifex aeolicus dimethylallyl diphosphate + NAD(P)+ + H2O
-
 r
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ formation of a mixture of isopentenyl diphosphate and dimethylallyl diphosphate in a ratio 1:5 Aquifex aeolicus isopentenyl diphosphate + NAD(P)+ + H2O
-
 r
1.17.7.4 additional information the IspH-catalyzed reaction involves formation of organometallic species, containing Fe-C bonds Aquifex aeolicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.7.4 HMBPP reductase
-
 Aquifex aeolicus
1.17.7.4 ispH
-
 Aquifex aeolicus
1.17.7.4 LytB
-
 Aquifex aeolicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.17.7.4 0.0091
-
 pH not specified in the publication, temperature not specified in the publication Aquifex aeolicus 2-(pyridin-3-yl)ethyl diphosphate
1.17.7.4 0.149
-
 pH not specified in the publication, temperature not specified in the publication Aquifex aeolicus pyridin-4-ylmethyl trihydrogen diphosphate
1.17.7.4 1.2
-
 pH not specified in the publication, temperature not specified in the publication Aquifex aeolicus pyridin-2-ylmethyl diphosphate

General Information

EC Number General Information Comment Organism
1.17.7.4 physiological function IspH is a 4Fe-4S protein that carries out an essential reduction step in isoprenoid biosynthesis Aquifex aeolicus