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Literature summary extracted from

  • Engstroem, K.; Nyhlen, J.; Sandstroem, A.G.; Baeckvall, J.E.
    Directed evolution of an enantioselective lipase with broad substrate scope for hydrolysis of alpha-substituted esters (2010), J. Am. Chem. Soc., 132, 7038-7042.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 expressed in Pichia pastoris strain X33 Moesziomyces antarcticus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.3 F149Y/I150N/F233G the mutant shows (R)-selectivity Moesziomyces antarcticus
3.1.1.3 F233G the mutant shows (R)-selectivity and a 30fold increase in activity for most substrates compared to the wild type enzyme Moesziomyces antarcticus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Moesziomyces antarcticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl 2-(4-methylphenyl)propanoate + H2O 55.6% conversion, (S)-selectivity Moesziomyces antarcticus 4-nitrophenol + 2-(4-methylphenyl)propanoate
-
 ?
3.1.1.3 4-nitrophenyl 2-benzylpropanoate + H2O 80.3% conversion, (S)-selectivity Moesziomyces antarcticus 4-nitrophenol + 2-benzylpropanoate
-
 ?
3.1.1.3 4-nitrophenyl 2-ethylhexanoate + H2O 75.3% conversion, (S)-selectivity Moesziomyces antarcticus 4-nitrophenol + 2-ethylhexanoate
-
 ?
3.1.1.3 4-nitrophenyl 2-methylheptanoate + H2O 80.7% conversion, (S)-selectivity Moesziomyces antarcticus 4-nitrophenol + 2-methylheptanoate
-
 ?
3.1.1.3 4-nitrophenyl 2-phenylbutanoate + H2O 17% conversion, (R)-selectivity Moesziomyces antarcticus 4-nitrophenol + 2-phenylbutanoate
-
 ?
3.1.1.3 4-nitrophenyl 2-phenylpentanoate + H2O 88.1% conversion, (R)-selectivity Moesziomyces antarcticus 4-nitrophenol + 2-phenylpentanoate
-
 ?
3.1.1.3 4-nitrophenyl 2-phenylpropanoate + H2O 38% conversion, (S)-selectivity Moesziomyces antarcticus 4-nitrophenol + 2-phenylpropanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 CalA
-
 Moesziomyces antarcticus
3.1.1.3 lipase A
-
 Moesziomyces antarcticus