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Literature summary extracted from

  • Fay, A.W.; Blank, M.A.; Lee, C.C.; Hu, Y.; Hodgson, K.O.; Hedman, B.; Ribbe, M.W.
    Characterization of isolated nitrogenase FeVco (2010), J. Am. Chem. Soc., 132, 12612-12618.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.6.1 strain YM68A expresses the His-tagged VFe protein, strain YM13A expresses the His-tagged MoFe protein, and strain DJ1143 expresses the His-tagged DELTAnifB MoFe protein Azotobacter chroococcum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.1 Molybdenum in the FeMoco cofactor of the MoFe protein Azotobacter chroococcum
1.18.6.1 Vanadium in the FeVco cofactor Azotobacter chroococcum

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Azotobacter chroococcum
-
 contains Mo- and V-nitrogenases
-
1.18.6.1 Azotobacter chroococcum YM68A
-
 contains Mo- and V-nitrogenases
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.6.1 partially, extraction of FeVco in an active form by N-methylformamide, and gel filtration of the reconstituted protein, overview Azotobacter chroococcum

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.18.6.1 native enzyme is reconstituted from N-methylformamide extraction Azotobacter chroococcum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 additional information FeVco is extracted intact, carrying with it the characteristic capacity to reduce C2H2 to C2H6 and, perhaps even more importantly, the ability to reduce N2 to NH3 Azotobacter chroococcum ?
-
 ?
1.18.6.1 additional information FeVco is extracted intact, carrying with it the characteristic capacity to reduce C2H2 to C2H6 and, perhaps even more importantly, the ability to reduce N2 to NH3 Azotobacter chroococcum YM68A ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.18.6.1 Mo-nitrogenase
-
 Azotobacter chroococcum
1.18.6.1 molybdenum-nitrogenase
-
 Azotobacter chroococcum
1.18.6.1 nitrogenase FeVco
-
 Azotobacter chroococcum
1.18.6.1 V-nitrogenase
-
 Azotobacter chroococcum
1.18.6.1 vanadium-nitrogenase
-
 Azotobacter chroococcum

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.6.1 FeMo cofactor i.e. FeMoco cofactor, in the Mo-nitrogenase Azotobacter chroococcum
1.18.6.1 iron-vanadium cofactor i.e. FeVco cofactor, in the V-nitrogenase Azotobacter chroococcum
1.18.6.1 additional information the cofactors of the Mo- and V-nitrogenases, i.e. FeMoco and FeVco, are homologous metalccenters with distinct catalytic properties, differences in electronic properties and structural topology, overview Azotobacter chroococcum