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Literature summary extracted from

  • Lee, H.Y.; Chung, H.S.; Hang, C.; Khosla, C.; Walsh, C.T.; Kahne, D.; Walker, S.
    Reconstitution and characterization of a new desosaminyl transferase, EryCIII, from the erythromycin biosynthetic pathway (2004), J. Am. Chem. Soc., 126, 9924-9925.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.278 medicine potential use to make unnatural macrolides for use as antibacterial Saccharopolyspora erythraea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.278 coexpression of EryCIII with the GroEL/ES chaperone complex greatly enhances the expression of soluble EryCIII protein Saccharopolyspora erythraea

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.278 Saccharopolyspora erythraea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.278
-
 Saccharopolyspora erythraea

Storage Stability

EC Number Storage Stability Organism
2.4.1.278 4°C, activity of concentrated enzyme stored overnight is more than 3 times lower than that of freshly purified enzyme. Saccharopolyspora erythraea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.278 dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 3-alpha-mycarosylerythronolide B TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose i.e. TDP-D-desosamine Saccharopolyspora erythraea dTDP + erythromycin D product detected by HPLC. Identity is conformed via mass spectrometry and chromatographic comparison with an authentic reference standard ?
2.4.1.278 additional information no activity with: TDP-D-mycaminose, 6-deoxy-erythronolide B, 10-deoxymethynolide Saccharopolyspora erythraea ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.278 EryCIII
-
 Saccharopolyspora erythraea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.278 25
-
 assay at Saccharopolyspora erythraea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.278 1.7
-
 3-alpha-mycarosylerythronolide B pH 8.0, 25°C, in the presence og 0.001 mM EryC and 1 mM of each substrate, more than 300 turnovers are observed in 2.5 min, indicating that the kcat must be greater than 100/min. This is one of the highest turnover numbers reported for an antibiotic glycosyl transferase Saccharopolyspora erythraea
2.4.1.278 1.7
-
 dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose pH 8.0, 25°C, in the presence og 0.001 mM EryC and 1 mM of each substrate, more than 300 turnovers are observed in 2.5 min, indicating that the kcat must be greater than 100/min. This is one of the highest turnover numbers reported for an antibiotic glycosyl transferase Saccharopolyspora erythraea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.278 8
-
 assay at Saccharopolyspora erythraea