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Literature summary extracted from

  • Lin, J.; Pantalone, V.R.; Li, G.; Chen, F.
    Molecular cloning and biochemical characterization of an endo-beta-mannanase gene from soybean for soybean meal improvement (2011), J. Agric. Food Chem., 59, 4622-4628.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Escherichia coli Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Mn2+ 5 mM, 50% residual activity Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.78 additional information
-
 locust bean gum KM value is 34.9 mg/ml, pH 5.0, 28°C Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.78 additional information Glycine max enzyme is active in hydrolyzing the beta-mannan-rich cell wall of soybean seeds ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Glycine max
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.78 cotyledon specifically expressed in cotyledons of seedling Glycine max
-
3.2.1.78 seedling specifically expressed in cotyledons of seedling Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 locust bean gum + H2O
-
 Glycine max ?
-
 ?
3.2.1.78 additional information enzyme is active in hydrolyzing the beta-mannan-rich cell wall of soybean seeds Glycine max ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 Man1
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 50
-
-
 Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 37
-
 stable up to Glycine max
3.2.1.78 50
-
 30 min, 20% residual activity Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.78 25.2
-
 locust bean gum pH 5.0, 28°C Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 5
-
-
 Glycine max