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Literature summary extracted from

  • Prima, V.; Wang, A.; Molina, G.; Wang, K.K.; Svetlov, S.I.
    Inhibition of LPS toxicity by hepatic argininosuccinate synthase (ASS): Novel roles for ASS in innate immune responses to bacterial infection (2011), Int. Immunopharmacol., 11, 1180-1188.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.5 lipopolysaccharide argininosuccinate synthase accumulates in circulation within 1 h after treatment with both lipopolysaccharide alone and hepatotoxic combination of lipopolysaccharide and D-Galactosamine. Enzyme physically binds to lipopolysaccharide Mus musculus
6.3.4.5 lipopolysaccharide argininosuccinate synthase accumulates in circulation within 1 h after treatment with both lipopolysaccharide alone and hepatotoxic combination of lipopolysaccharide and D-Galactosamine. Enzyme physically binds to lipopolysaccharide Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.5 expression in Escherichia coli Mus musculus
6.3.4.5 expression in Escherichia coli Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.5 47000
-
 x * 47000, calculated for His-tagged recombinant protein Mus musculus
6.3.4.5 47000
-
 x * 47000, calculated for His-tagged recombinant protein Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.5 Homo sapiens P00966
-
-
6.3.4.5 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.5 blood
-
 Mus musculus
-
6.3.4.5 liver
-
 Mus musculus
-
6.3.4.5 serum argininosuccinate synthase accumulates in circulation within 1 h after treatment with both lipopolysaccharide alone and hepatotoxic combination of lipopolysaccharide and D-Galactosamine Homo sapiens
-

Subunits

EC Number Subunits Comment Organism
6.3.4.5 ? x * 47000, calculated for His-tagged recombinant protein Mus musculus
6.3.4.5 ? x * 47000, calculated for His-tagged recombinant protein Homo sapiens

General Information

EC Number General Information Comment Organism
6.3.4.5 physiological function recombinant argininosuccinate synthase reduces lipolysaccharide cytotoxicity, TNF-alpha production, and increases cell viability in cultured mouse macrophages, even when added one h following lipopolysaccharide challenge. Intraperitoneal injection of recombinant argininosuccinate synthase at 5 mg/kg after treatment with a high dose of lipopolysaccharide remarkably increases survival of rodents, with a concomitant decrease of sepsis markers TNF-alpha, C-reactive protein, and lactate dehydrogenase levels in blood Mus musculus